用丙硫苯咪唑检测羊线虫抗药性的体外虫卵孵化试验  

An egg hatch assay using albendazole for in vitro detection of resistance of sheep and goat nematodes to anthelmintics

作　　者：蔡葵蒸[1] 杨晓野[1] 杨春生 郝成[1] 王晓亮 梁小军[3] 王瑞[1] 张晓东[1] 张玉玲 李昕 韩海宾[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]宁夏动物防疫站,宁夏银川750002 [3]宁夏农林科学院,宁夏银川750002

出　　处：《中国兽医科学》2007年第8期721-725,共5页Chinese Veterinary Science

摘　　要：为探讨用丙硫苯咪唑进行体外虫卵孵化试验检测羊线虫对苯并咪唑类药物的抗药性,对23个羊场的25份田间样品用丙硫苯咪唑和噻苯咪唑进行了虫卵孵化试验并与先前减卵试验（faecal egg count re-duction test,FECRT）的结果比较。结果表明,25份样品中,丙硫苯咪唑和噻苯咪唑对受检虫卵的半数致死量（LD50）均值分别为0.050 1和0.054 0μg/mL,差异不显著。FECRT检测有抗药性的4个羊场,75%的样品对丙硫苯咪唑和噻苯咪唑的LD50均值均在0.1μg/mL以上,只有1个羊场的的LD50值分别为0.068 9μg/mL（丙硫苯咪唑）和0.071 2μg/mL（噻苯咪唑）。检测为可疑的2个羊场,其样品的LD50值为0.04～0.07μg/mL;FECRT检测敏感的蠕虫群体的LD50均在0.04μg/mL以下。此外,丙硫苯咪唑用纯的二甲基亚砜溶解和稀释后于4℃保存7 d,LD50值变化不大,提示药效无明显下降,而保存14 d后药效有下降趋势。To detect resistance of nematodes in （ALB）, 25 samples collected from 23 farms were sheep and goats to benzimidazoles（BZs） by albendazole examined simultaneously by ALB and thiabendazole （TBZ） in egg hatch assay（EHA）,and the comparison of resistance-to-BZs was carried out between faecal egg count reduction test（FECRT）and EHA. The mean LD50 of ALB and TBZ in all samples were 0. 050 1μg/mL and 0. 054 0μg/mL,respectively,and there was no significant difference between them. On 4 farms where resistance-to-BZs was detected by FECRT, the mean LD50 values were over 0. 1 μg/mL in 75% of samples for ALB and TBZ,and the LD50 values were 0. 068 9 μg/mL for ALB and 0. 071 2μg/mL for TBZ on 1 farm out of the 4 farms. On another 2 farms where the suspected isolates were detected by FECRT, and the LD50 values ranged from 0.04 to 0. 07μg/mL. In worm populations which were susceptible to BZs in FECRT,the LD50 values were lower than 0.04μg/mL. In addition, the effect of DMSO-dissolved ALB solution which had been stored at 4 ℃ for 7 days on the LD50 values in vitro was not significant,indicating that the efficacy of ALB in solvent was steady,but its efficacy declined after 14 days of storage.

关 键 词：驱虫药抗性 丙硫苯咪唑 虫卵孵化试验 粪便虫减少试验 绵羊 山羊 线虫 

分 类 号：S859.795[农业科学—临床兽医学] S852.731[农业科学—兽医学]