肝再生增强因子在隐睾生精细胞中的表达及意义  

Expression of Augmenter of Liver Regeneration in Cryptorchidism Spermatogenic Cells and Its Implication

作　　者：陈旋[1] 张德迎[2] 刘星[2] 魏光辉[2] 

机构地区：[1]浙江大学医学院附属儿童医院麻醉科,浙江杭州310003 [2]重庆医科大学附属儿童医院泌尿外科,重庆400014

出　　处：《中华男科学杂志》2007年第8期700-705,共6页National Journal of Andrology

摘　　要：目的:探讨肝再生增强因子(ALR)在隐睾生精细胞中的表达及其意义。方法:建立SD大鼠隐睾模型(n=20),设立正常(n=10)和手术对照(n=10)。分别采用免疫组化法、免疫荧光法检测术后10、40d(即PND30和PND60)大鼠睾丸组织生精细胞ALR和细胞色素C氧化酶亚单位Ⅱ(COXⅡ)表达情况;Perls染色法检测睾丸中三价铁含量。结果:正常和手术对照组精原细胞ALR呈强表达,隐睾组精原细胞ALR表达显著减弱;同年龄段各组间两两比较,COXⅡ表达水平差异无显著性;术后10d(PND30)隐睾组三价铁含量较同年龄段正常对照组和手术对照组显著减少(P<0.05)。结论:ALR在生精细胞发育的早期起着重要作用,隐睾ALR缺陷所引起的代谢障碍可能是其精子发生障碍的重要机制之一。Objective： To investigate the expression of the augmenter of liver regeneration （ALR） in cryptorchidism spermatogenic cells. Methods： Twenty cryptorchidism rat models were established by surgery, 10 included as normal controls and anther 10 as sham-surgery controls. The expression of ALR was detected by immunochemistry, the COXⅡ level measured by immunofluorescence and the ferric iron contents assayed by Perl＇s stain. Results ： ALR expressed intensively in the spermatogonia of the control groups but in a signigicantly diminished manner in the cryptorchidism group. No significant difference was found in the COXⅡ level between any two groups of the same age. Ferric iron content of the PND30 cryptorchids decreased significantly （ P 〈 0.05 ）. Conclusion ： ALR may play an important role in early spermatogenesis. Metabolism dysfunctions caused by ALR defection might be a crucial mechanism for aspermatogenesis of cryptorchidism.

关 键 词：隐睾 肝再生增强因子 精子发生 线粒体 铁硫簇 大鼠 

分 类 号：R697.2[医药卫生—泌尿科学]