大鼠神经元液压伤后依钙蛋白酶的时程变化及亚低温的影响  

Time course of calpain activity changes in rat neurons following fluid percussion injury and the interventional effect of mild hypothermia

作　　者：隋立森[1] 韩富[1] 郭燕舞[2] 姜晓丹[2] 徐如祥[2] 刘恩重[3] 张相彤[3] 戴钦舜[3] 

机构地区：[1]广州中医药大学第二临床医学院神经外科,广东广州510120 [2]南方医科大学珠江医院神经外科,广东广州510282 [3]哈尔滨医科大学附属第一医院神经外科,黑龙江哈尔滨150001

出　　处：《南方医科大学学报》2007年第8期1149-1151,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30070270)~~

摘　　要：目的研究常温时液压冲击伤后神经元内依钙蛋白酶(Calpain)活性变化及亚低温的干预机制。方法培养大鼠神经元并制作液压冲击伤模型,在不同时间点进行亚低温干预,以紫外分光光度法检测液压冲击伤后的神经元内Calpain活性变化及在不同时段亚低温对Calpain活性的干预效应。结果常温时,在细胞损伤后Calpain的活性发生显著变化。亚低温组与常温组Calpain的活性不同,且亚低温对伤后Calpain活性的干预效应与作用起始时间密切相关。结论液压冲击伤后神经元内Calpain活性发生的改变参与细胞创伤的病理过程,亚低温可能通过这一机制具有一定保护创伤性脑损伤的作用。亚低温干预的效果与开始干预时间相关。Objeetive To investigate the time course of calpain activity changes in rat neurons following fluid percussion injury （FPI） under normothermia （37℃） and mild hypothermia （32 0.5） ℃. Methods In vitro cultured rat neurons were subjected to FPI followed by application of mild hypothermia for intervention at different time points, and the changes in intraneuronal calpain activity following FPI and the interventional effect of mild hypothermia on calpain activity were evaluated by UV-spectrophotometry at different time points. Results Remarkable changes occurred in calpain activity in the neurons following FPI at 37 ℃, and mild hypothermia produced obvious interventional effect on calpain activity in＇close relation to the timing of intervention initiation. Conclusion Intraneuronal calpain activity changes following FPI are involved in the pathological process of cellular injury, and mild hypothermia might offer protection against traumatic brain injury to some extent by regulating calpain activity. The interventional effect of mild hypothermia is associated with the timing of the intervention initiation.

关 键 词：神经元 液压冲击伤 依钙蛋白酶 亚低温 

分 类 号：R651.3[医药卫生—外科学]