白菜雄性不育相关基因BcMF3和BcMF4的A9启动子的基因沉默植物表达载体的构建  

Construction of Gene Silencing Plant Expression Vectors of BcMF3 and BcMF4 Genes Related to Male Sterility in Chinese Cabbage-pak-choi (Brassica campestris ssp.chinensis)

作　　者：刘乐承[1] 曹家树[1] 

机构地区：[1]浙江大学蔬菜研究所

出　　处：《长江大学学报（自科版）（中旬）》2007年第2期48-53,共6页Journal of Yangtze University(Nature Science Edition)

基　　金：国家自然科学基金项目(30370975);浙江省重大科技项目(2005C12019-02)

摘　　要：以白菜(Brassica campestris ssp.chinensis,syn.B.rapa ssp.chinensis)雄性不育相关基因BcMF3和BcMF4为沉默对象,利用绒毡层组织特异性的A9启动子,构建了反义RNA、RNA干涉和双价反义RNA植物表达载体。结果表明,经过一系列分子操作,获得了A9启动子和目的基因片段,并利用它们构建了5个基因沉默植物表达载体,可用于基因功能验证。Targeting at BcMF3 and BcMF4 genes,which related to male sterility of Chinese cabbage-pak-choi (Brassica campestris ssp.chinensis var.communis,syn.B.rapa ssp.chinensis var.communis),gene silencing vectors of antisense RNA,RNAi (RNA interference) and bivalent antisense RNA were constructed using tapetum-specific A9 promoter.The results indicated that,after a serial molecular manipulation,A9 promoter and target gene fragments were obtained.Subsequently 5 gene silencing plant expression vectors were constructed.These vectors can be used in future functional validation of the two genes.

关 键 词：白菜 雄性不育 BcMF3 BcMF4 基因沉默 表达载体 

分 类 号：Q784[生物学—分子生物学] S634.3[农业科学—蔬菜学]