伪鹅观草高分子量麦谷蛋白基因启动子的克隆  

Cloning the Promoter of High Molecular Weight Glutenin Subunit Gene in Pseudoroegneria stipifolia

作　　者：李志新[1] 曹双河[2] 张相岐[2] 张怀刚[3] 

机构地区：[1]长江大学农学院,湖北荆州434025 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]中国科学院西北高原生物研究所,青海西宁810008

出　　处：《长江大学学报（自科版）（中旬）》2007年第2期57-59,80,共4页Journal of Yangtze University(Nature Science Edition)

基　　金：国家自然科学基因资助项目(30521001);中国科学院资助项目(CXLY-2002-6)

摘　　要：通过PCR克隆的方法,从二倍体伪鹅观草St基因组中克隆到了高分子量麦谷蛋白亚基基因Glu-1St1的启动子,该序列全长959bp,序列比对及结构分析表明,它与麦类高分子量麦谷蛋白亚基基因的启动子序列的一致性很高,达到82%,包含有典型麦谷蛋白亚基基因启动子的顺式作用元件。系统进化分析表明,它与大麦的D-hordein基因启动子的进化关系比较近,属于典型的x型亚基基因的启动子。The promoter sequence with 959 bp of Glu-1St1 was obtained by genomic PCR experiment in diploid Pseudoroegneria stipifolia which contained St genome.The structural analysis indicated that the sequence had very high identity with the promoters of HMW-GS(high molecular weight glutenin subunits) genes in Triticum,and including an identical set of cis-elements found in promoter regions of previously characterized wheat HMW-GS genes.Phylogenetic analysis indicated that the promoter of Glu-1St1 was closely related with that of D-hordein,and clustered to x-type subunit gene promoters.

关 键 词：中文关键词 高分子量麦谷蛋白亚基基因 启动子 伪鹅观草(Pseudoroegneria stipifolia StSt) 克隆 

分 类 号：Q785[生物学—分子生物学]