黄连肉桂药对配伍对黄连总碱大鼠小肠吸收的影响  

Influence of match-pair of huanglian-rougui on rat intestinal absorption of total huanglian alkali

作　　者：张玉杰[1] 杨洁[1] 邹晓翠[1] 刘丽梅[2] 孙万晶[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国中医科学院基础理论研究所,北京100700

出　　处：《中国中药杂志》2007年第15期1521-1524,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672585)

摘　　要：目的:考察黄连总碱大鼠小肠吸收特性及黄连肉桂配伍对黄连总碱小肠吸收的影响,探讨黄连肉桂药对配伍规律。方法:采用大鼠体外小肠吸收模型,考察时间、浓度及转运方向对黄连总碱大鼠小肠摄取和转运的影响;同时考察黄连与肉桂不同比例配伍后,黄连总碱的小肠吸收和转运的变化规律;采用薄层色谱法对黄连肉桂水煎液、小肠浆膜液、组织匀浆液中的总碱成分进行定性鉴别。结果:黄连水煎液中总碱成分具有与小檗碱类似的P-糖蛋白底物转运特性:总碱在大鼠小肠的摄取和转运具有浓度及时间依赖性;总生物碱的转运明显受到转运方向的影响,从浆膜面到黏膜面的转运明显大于从黏膜面到浆膜面。黄连与肉桂在一定配比范围内,黄连总碱的小肠吸收呈现明显增加的趋势,以黄连总碱为指标,二者配伍对黄连总碱的吸收存在最佳配比关系。黄连各生物碱成分经小肠吸收和转运特性相似。结论:小肠吸收和药物间的化学反应共同影响了黄连肉桂药对的配伍作用。Objective： To research the principle of match-pair of huanglian-rougui. Method： A rat intestinal model in vitro had been applied to investigate the absorption and transport characteristics of total huanglian alkali across intestinal mucosa, as well as the influence of match-pair of huanglian-rougui on its intestinal absorption. The identification of alkalis in primitive solution, serosal solution and intestinal homogenate were analyzed by TLC. Result： The transport and uptake characteristic of total alkali in huanglian solution was similar to berberine. The uptake and transport of total alkali across intestinal mucosa was concentration-and time-dependent. Transport directions had strong effects on the transport of total alkali. The amount of total alkali transport from serosal side to mucosal side was much more than that from mucosal side to serosal side. After matching with rougui as some ration, the intestinal absorption of total alkali increased significantly comparing with huanglian alone, and there was an optimal ration among match-pair of huanglianrougui. Conclusion： Chemical reaction in vitro and intestinal absorption were the key factors of match-pair of huanglian-rougui.

关 键 词：黄连肉桂药对配伍 小肠吸收 黄连总碱 

分 类 号：R285.5[医药卫生—中药学]