慢性阻塞性肺疾病大鼠主要脏器γ-GCS活性及其mRNA表达变化  

The activity and expression of γ-glutamylcsteine synthetase in major organs of rats with chronic obstructive pulmonary disease

作　　者：林书典 戴爱国 

机构地区：[1]湖南省老年医院-湖南省老年医学研究所呼吸病研究室,长沙410001

出　　处：《心肺血管病杂志》2007年第3期161-164,169,共5页Journal of Cardiovascular and Pulmonary Diseases

基　　金：湖南省科技厅科研基金项目(01JZY2017)

摘　　要：目的:研究慢性阻塞性肺疾病(COPD)大鼠血浆、肺、心、肝、和肾组织中氧化/抗氧化水平,及γ谷氨酰半胱氨酸合成酶(γ-GCS)活性及其表达在各器官组织中的差异和变化。方法:健康雄性Wistar大鼠14只,随机分COPD模型组和对照组,每组7只。采用每日熏香烟和两次气管内滴入脂多糖(LPS)法制作COPD大鼠模型。检测大鼠血浆、肺、心、肝和肾组织中还原型谷胱甘肽(GSH)、活性氧(ROS)、总抗氧化力(T-AOC)和γ-GCS活性。用逆转录-聚合酶链反应(RT-PCR)法检测肺、心、肝和肾组织中γ-GCS mRNA的表达。结果:COPD组大鼠心、肝中GSH、ROS、T-AOC和γ-GCS活性均显著增高(P均<0.05),但未显示出明显氧化/抗氧化失衡。肺中GSH和ROS提高,而T-AOC下降明显(P均<0.05),表明提高的GSH不足以抵御氧化作用,明显存在氧化/抗氧化失衡。血清中ROS增高,GSH和T-AOC下降明显,显示系统性氧化/抗氧化失衡。COPD大鼠肺组织γ-GCS mRNA表达较对照组显著增高(P<0.05),而心、肝、肾组织中γ-GCS mRNA表达与对照组无明显差异(P>0.05)。结论:COPD大鼠肺内存在氧化/抗氧化失衡,肺组织是γ-GCS表达的主要部位,在抗氧化损伤中可能发挥重要作用。Objective：To investigate the activity and expression of γ-glutamylcsteine synthetase （γ-GCS）, and the changes of oxidative/antioxidative status in plasma, lung, heart, liver and renal tissue of chronic obstructive pulmonary disease （COPD） rats, to study the rale of γ-GCS play in COPD rats. Method： Fourteen male Wistar rats were randomly divided into COPD model group and control group. The rat COPD model was established by intratracheal instillation of lipopolysaccharide （LPS 1 mg/mL） 200/A twice and exposure to cigarette smoke daily. The levels of glutathione （ GSH ）, reactive oxygen species （ ROS）, total antioxygen capacity （ T-AOC ）, and the activity of γ-GCS in plasma, lung, heart, liver and renal tissues of rats were measured. Reverse transcription- polymersde chain reaction （RT-PCR） was used to determined the expression of γ-GCS in lung, heart, liver and renal tissues of rats. Result：The levels of GHS, ROS, T-AOC and γ-GCS activity in heart and liver of rats were higher in COPD group compare with their control group （all P 〈 0.05 ）, however, there was no evidence for oxidative/ antixoidative imbalance. GSH level raised as well as ROS did, T-AOC was lower in COPD group compare with their control group （all P 〈 0.05）, RT-PCR analysis showed that the expression of γ-GCS mRNA was higher in lung of rats in COPD group compare with their control group （ P 〈 0.05）, while there were no significant difference in heart, liver and renal tissues between two groups. Conclusion：There was oxidative/antioxidative imbalance in lung tissue in COPD rat model, the lung may be the main organ for γ-GCS expression, and play an important role in antioxidative course.

关 键 词：Γ谷氨酰半胱氨酸合成酶 肺疾病 慢性阻塞性 氧化/抗氧化 动物实验 大鼠 

分 类 号：R563[医药卫生—呼吸系统]