高效液相色谱法分析人胎肝细胞培养液中7-酮基胆固醇  

HPLC determination of 7 ketocholesterol in human fetal liver cell culture medium

作　　者：张庆林[1] 吴祖泽[1] 曹菊荣[1] 薛惠华[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1997年第2期128-130,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：建立了高效液相色谱法分析人胎肝细胞培养液中７－酮基胆固醇。人胎肝细胞培养液经氯仿－甲醇（体积比为２∶１）混合液提取后，水洗，氮气吹干。色谱条件为色谱柱：μＰｏｒａｓｉｌＳｉＯ２，流动相：正己烷∶异丙醇（体积比为９１∶９），２３３ｎｍ检测。这一方法具有快速，灵敏度高，重复性好等特点。A fast, sensitive, high performance liquid chromatographic method was developed for determination of 7 ketocholesterol in human fetal liver cell culture medium. In this procedure 2∶1 chloroform∶methanol (V/V) extracts of human fetal liver cell culture medium were combined, washed with water and evaporated to dryness under N 2. HPLC conditions included μ Porasil SiO 2 column, mobile phase: hexane∶isopropanol (91∶9), detected at 233 nm. This method was successfully applied to human fetal liver cell culture medium.

关 键 词：细胞培养 7-酮基胆固醇 高效液相色谱 胎肝 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学] R329-33[医药卫生—基础医学]