交替拟合残差算法与HPLC-DAD结合快速测定复合麻醉剂  

Fast Determination of Compound Anaesthetic Drugs Using AFR Algorithm Combined with HPLC-DAD

作　　者：丁玉洁[1] 吴海龙[1] 夏阿林[1] 崔卉[1] 方冬梅[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室分析测试中心,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2007年第8期54-56,共3页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(2047501420435010)

摘　　要：将交替拟合残差算法与HPLC-DAD（高效液相色谱-二极管阵列检测）相结合,用于复合麻醉剂中盐酸普鲁卡因和盐酸利多卡因的同时直接快速测定,两组样本中盐酸利多卡因的回收率范围分别为96.2%-101.6%和100.3%-102.3%,盐酸普鲁卡因的回收率范围分别为93.7%-110.4%和97.3%-107.9%.研究表明,交替拟合残差算法可以很好地解决HPLC中色谱和光谱的重叠问题。Procaine and lidocaine of compound anaesthetic drugs have been determined simultaneously by using the alternating fitting residue （AFR） method coupled with HPLC-DAD. In the two groups of data, the recovery ranges of procaine and lidocaine were 96.2 % - 101.6 % and 100.3 % - 102.3 % , 93.7 % - 110.4 % and 97.3 % - 104.0 %, respectively. The satisfying results have proved that AFR algorithm can solve the problem of overlapped chromatograms and spectra peaks.

关 键 词：交替拟合残差算法 HPLC-DAD 利多卡因 普鲁卡因 

分 类 号：O657[理学—分析化学]