高原红细胞增多症患者骨髓单个核细胞GATA-1和GATA-2的表达研究  

Expression of GATA-1 and GATA-2 in the bone marrow of patients with Monge's disease

作　　者：李建平[1] 贾乃镛[1] 李占全[1] 王红心[1] 苏娟[1] 李玉丽[1] 李学梅[1] 

机构地区：[1]青海大学附属医院血液科,西宁810001

出　　处：《中华血液学杂志》2007年第8期537-540,共4页Chinese Journal of Hematology

摘　　要：目的探讨高原红细胞增多症患者骨髓单个核细胞(MNC)GATA-1、GATA-2 mRNA 及蛋白表达的变化及作用。方法采用 RT-PCR 法检测高原红细胞增多症患者骨髓 MNC GATA-1、GATA-2 mRNA 表达水平,并采用免疫细胞化学方法检测骨髓细胞 GATA-1、GATA-2蛋白水平。同时以13例非血液病患者及7名正常人为对照。结果①高原红细胞增多症患者 GATA-1和 GATA-2mRNA 的相对表达水平分别为1.033±0.146和0.451±0.073,对照组 GATA-1和 GATA-2相对表达水平分别为0.458±0.076和0.185±0.074,高原红细胞增多症患者明显高于对照组(P<0.01)。免疫细胞化学检测结果显示高原红细胞增多症患者 GATA-1和 GATA-2蛋白表达均为阳性,阳性细胞数分别为77.3±33.3和29.4±11.4,阳性细胞积分分别为135.4±75.4和48.4±19.7。20例对照均表达GATA-1蛋白(阳性细胞数为18.1±11.3,阳性细胞积分为24.2±13.4),仅12例表达 GATA-2蛋白(阳性细胞数为5.4±3.0,阳性细胞积分为7.3±4.2),高原红细胞增多症患者明显高于对照组(P<0.01)。②GATA-1、GATA-2 mRNA 与蛋白表达水平呈正相关(P<0.01),高原红细胞增多症患者GATA-1 mRNA 和蛋白表达水平与血红蛋白浓度呈正相关(P<0.01)。③GATA-1和 GATA-2蛋白表达分布在细胞质而非细胞核。结论高原红细胞增多症患者骨髓细胞中 GATA-1、GATA-2表达升高,进一步影响红系血红蛋白基因表达增强,可能是高原红细胞增多症的重要发病机制之一。Objective To find out how GATA-1 and GATA-2 behave in the bone marrow of patients with Monge＇ s disease. Methods The levels of mRNA in mononuclear cells （MNC） and proteins of GATA-1 and GATA-2 in the bone marrow of patients with Mange＇s disease and controls were determined by RT-PCR and immune cytolysis chemical method. Results ①All patients and controls expressed GATA-1 mRNA （ Monge＇ s disease 1. 033 ± 0. 146, Control 0.458 ± 0. 076） and GATA-2 mRNA （ Monge＇s disease 0.451 ± 0. 073, Control 0. 185 ± 0. 074）. All patients expressed both GATA-1 （positive cell counts 77.3 ± 33.3, positive score 135.4 ± 75.4） and GATA-2 （ positive cell counts 29.4 ± 11.4, positive score 48.4 ± 19.7）. All the controls expressed GATA-1 （ positive cell counts 18.1 ± 11.3, positive score 24. 2 ± 13.4） while 12 of 20 controls expressed GATA-2（ positive cell counts 5.4 ± 3.0, positive score 7.3 ±4.2 ）. The expression of mRNA and proteins of GATA-1 and GATA-2 in Monge＇ s disease were higher than in controls （P 〈0.01 ）. ②There was a positive correlation between GATA-1 and Hb （P 〈 0.01 ）, as did between mRNA and proteins of GATA-1 and GATA-2. ③Both the proteins of GATA-1 and GATA-2 located only in the cytoplasm but not the nucleus. Conclusions Two of inherent genes, GATA-1 and GATA-2 which were expressed at higher levels in patients with Monge＇s disease than in controls might play significant roles in the pathogenesis of Monge＇s disease.

关 键 词：高原病 转录因子 GATA 

分 类 号：R55[医药卫生—血液循环系统疾病]