高效液相色谱-电喷雾质谱(HPLC-ESI-MS/MS)测定新型抗癌铂配合物  

Application of HPLC-ESI-MS/MS to the Determination of a Novel Antitumour Platinum Complex

作　　者：苏强[1] 刘清飞[2] 梁琼麟[3] 王义明[2] 毕开顺[1] 刘伟平[4] 罗国安[2] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]清华大学生命科学与医学研究院,北京100084 [3]清华大学化学系,北京100084 [4]昆明贵金属研究所,昆明650221

出　　处：《高等学校化学学报》2007年第8期1486-1488,共3页Chemical Journal of Chinese Universities

基　　金：清华大学裕元医学研究基金(批准号:20240000529)资助

摘　　要：A novel platinum complex,named as saliplatin,was designed,synthesized and characterized with element analysis,coupled inductively plasma-mass spectrum,gravitational thermal-differential thermal analysis,ultraviolet-visible absorption spectrum,infrared spectrum,fast atom bombment mass spectrum and 1H NMR,etc.Its purity was investigated and determined.HPLC-ESI-MS was employed to separate the platinum complex and the impurity,and to identify and quantify the latter.It proved that the impurity in this novel compound was 3,5-diisopropylsalycilic acid,a reactant used in the synthesis of the novel platinum complex,identified with ESI-MS.The mass fraction was determined with HPLC as 4.3%,which meant that the purity of this new substance was 95.7%.A novel platinum complex, named as saliplatin, was designed, synthesized and characterized with element analysis, coupled inductively plasma-mass spectrum, gravitational thermal-differential thermal analysis, ultraviolet-visible absorption spectrum, infrared spectrum, fast atom bombment mass spectrum and I H NMR, etc. Its purity was investigated and determined. HPLC-ESI-MS was employed to separate the platinum complex and the impurity, and to identify and quantify the latter. It proved that the impurity in this novel compound was 3,5-diisopropylsalycilic acid, a reactant used in the synthesis of the novel platinum complex, identified with ESI-MS. The mass fraction was determined with HPLC as 4. 3%, which meant that the purity of this new substance was 95.7%.

关 键 词：电喷雾质谱 铂配合物 抗癌药物 

分 类 号：O657.6[理学—分析化学]