生物陶粒反应器中好氧反硝化菌的脱氮研究  

Nitrogen removal by aerobic denitrifies in bio-ceramic reactor

作　　者：马放[1] 张佳[1] 张献旭[1] 魏利[1] 杨基先[1] 

机构地区：[1]哈尔滨工业大学环境科学与工程系,黑龙江哈尔滨150090

出　　处：《中国环境科学》2007年第4期529-533,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(50521140075);国家"973"项目(2004CB418505)

摘　　要：采用固定化生物强化技术，研究了生物陶粒反应器中好氧反硝化菌的脱氮效果．运用变性梯度凝胶电泳技术（DGGE）对微生物菌群结构稳定性进行了分析．结果表明，在水力负荷1．5m／h，气水比5：1，水温25～30℃，进水硝氮负荷为0．75-2．87kg／（m^3·d），COD浓度负荷为7．46～10．03kg/（m^3·d）的条件下，反应器稳定运行后，对硝氮的平均去除率可以达到93．10％，并且出水硝氮和亚硝氮的浓度一直保持在很低的水平，COD的平均去除率为97．50％．DO的消耗和硝酸盐还原主要发生在进水端的0-0．5m区域内，反应器中DO浓度始终处于较高的水平．根据DGGE指纹图谱显示．分离得到的4株好氧反硝化菌为假单胞菌属的不同的种，且在整个运行阶段稳定存在，始终为优势菌群．The effects of air/water ratio, hydraulic load, temperature and C/N ratio on nitrogen removal efficiency of aerobic denitrifies in bio-ceramic reactor were investigated. Denaturing Gradient Gel Electrophoresis （DGGE） was used to analyze the stability of microbial community structure. 93.10% of nitrate nitrogen removal efficiency and 97.50% of COD removal efficiency could be reached under conditions of hydraulic load 1.5m/h air/water ratio 5：1, temperature 25-30℃ nitrate nitrogen loading 0.75-2.87kg/（m^3· d）,COD loading 7.46-10.03kg/（m^3· d）. DO and nitrate nitrogen concentration were reduced at the region 0-0.5m from the influent, and DO was always kept at a high level for all experiment. The aerobic denitrifies strains were identified with 16S rDNA. The PCR-DGGE profiles showed that the isolated four strains belong to Pseudomonas and the preponderant populations were dominant population in aerobic denitrification.

关 键 词：好氧反硝化 变性梯度凝胶电泳技术 生物陶粒反应器 

分 类 号：X703[环境科学与工程—环境工程]