宫颈癌病理组织中HPV基因型别分析  

Analysis of HPV genotypes in paraffin-embedded tissue of cervical cancer

作　　者：梁彩红[1] 杨光[1] 崔金环[1] 胡唯唯[2] 陈华锦[1] 

机构地区：[1]广东省佛山市第一人民医院临床医学研究所,528000 [2]广东省佛山市第一人民医院病理科,528000

出　　处：《江西医学检验》2007年第4期299-300,294,共3页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的调查本地区子宫颈癌病理组织中HPV基因型别,为子宫颈癌HPV筛查方法的选择提供依据。方法应用可检测23种HPV基因型别的基因芯片法对61例经病理证实的子宫颈癌石蜡包埋组织进行HPV基因型别分析。结果在61例子宫颈癌组织标本中,共检出41例HPV阳性,阳性率为67.2%,其中HPV16型别26例,占HPV阳性病例的63.4%(26/41),HPV18型别12例,占HPV阳性病例的29.2%(12/41),HPV33型1例,不同基因型混合感染2例,分别为HPV16/18与HPV16/59型别混合感染各一例,各占HPV阳性病例的2.4%(1/41)。结论在本地区的61例子宫颈癌病理组织中,以16和18基因型最为常见,共占HPV阳性型别构成比的95.3%,说明本地区与子宫颈癌密切相关的HPV基因型别较为集中。Objective To investigate the distribution of HPV genotypes in paraffin-em- bedded tissues of cervical cancer in Foshan region. Methods 61 cervical cancer tissues were analyzed by genechip technique with 23 HPV genotypes. Results The total positive rate of HPV was 67% （41/61） in 61 cervical cancer tissues diagnosed by histopathology. Among 41 positive cases, HPV16 was 63.4% （26/41）, with 29.2% （12/41） of HPV18, 2.4% （1/41） of HPV33; 4.8% （2/41） of mixed genatypes in which 1 case was HPV16/18 and the other was HPV16/59 respetively. Conclusions HPV16 and HPV18, with 95.3% of HPV positive genotypes in the cervical cancer tissues, are most prevalent in Foshan region. The results suggest that cervical cancer exist concentrated HPV genotynes.

关 键 词：人乳头瘤病毒 基因型 基因芯片 宫颈癌 

分 类 号：R446.8[医药卫生—诊断学] R737.33[医药卫生—临床医学]