胶原蛋白-明胶支架材料修复大鼠坐骨神经缺损的研究  

An experimental study on repair of peripheral nerve gaps with collagen-gelatin scaffolds

作　　者：孟浩[1] 罗卓荆[1] 王亮[1] 冯林杰[1] 兰丽锋[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032

出　　处：《中华创伤骨科杂志》2007年第8期711-713,共3页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金资助项目（30572165）中国专利号：200310118994.9

摘　　要：目的制备胶原蛋白一明胶神经支架材料（CG材料）并研究其在修复大鼠10mm坐骨神经缺损实验中的疗效。方法以I型胶原蛋白、明胶通过冷冻干燥技术制备具有轴向微管结构的神经支架材料，用其桥接修复sD大鼠坐骨神经10mm缺损。术后16周分别行透射电镜，S-100、β-tubulin class Ⅲ、NFl60免疫荧光染色以及电生理检测，观察支架材料引导神经再生的疗效。结果制备的材料内部为孔径均匀且平行排列的微管结构，术后16周通过透射电镜和免疫荧光染色可见大量再生神经纤维，电生理检测神经传导速度及波幅接近自体神经移植。结论胶原蛋白一明胶支架材料能够有效的促进周围神经再生。Objective To observe the effect of collagen-gelatin scaffolds developed by us on the repair of a 10 mm defect of sciatic nerve in rats. Methods The scaffolds were made of I-collagen and gelatin by freeze-drying method. Twenty SD rats with a 10 mm defect of sciatic nerve were randomly divided into 2 equal groups. In experimental group, the nerve gaps were repaired by collagen-gelatin scaffolds, while in control group the defects were repaired by nerve auto-transplantation. Sixteen weeks after the operation, all the rats were observed by transmission electron microscope, S-100, β-tubulin class Ⅲ, NF160 immunohisto- logical staining and electrophysiological analysis to evaluate the effects of scaffold on nerve regeneration. Results All the cylinder scaffolds had microscopic channels inside which were arranged in a parallel manner, and uniform in pore size. Sixteen weeks after the operation, a great number of regenerated nerves were observed by transmission electron microscope and immunohistological staining. Both the nerve conduction velocity and wave amplitude in experimental group were similar to those in control group. Conclusion Collagen-gelatin scaffolds can improve nerve regeneration effectively.

关 键 词：支架 神经移位 神经再生 胶原 

分 类 号：R686[医药卫生—骨科学]