细胞移植的人嗅鞘细胞培养方法的研究  

Method of purification of human olfactory ensheathing cells for transplantation.

作　　者：唐勇[1] 吴燕峰[2] 沈慧勇[1] 

机构地区：[1]中山大学第二附属医院骨科,广州510120 [2]中山大学第二附属医院脐血库,广州510120

出　　处：《中国医药》2007年第10期577-578,共2页China Medicine

基　　金：国家自然科学基金(30271311)

摘　　要：目的探讨适用于细胞移植的人嗅鞘细胞(OECs)分离与培养的方法。方法无菌条件下取自愿捐献孕5个月流产胎儿的完整嗅球进行嗅鞘细胞提取分离、纯化,并对嗅鞘细胞进行免疫组化染色及纯度检测,观察其形态学特点,检测其免疫组化染色的阳性率。结果本方法分离、培养的嗅鞘细胞大多呈三角形、梭形,有些突起较长,生长活跃,增殖迅速,P75、胶质纤维酸性蛋白(GFAP)免疫组化染色呈阳性,嗅鞘细胞阳性率为95.3%。结论该方法简便易行且不影响细胞功能,适用于细胞移植的研究。Objective To establish an effective method of isolation and culture of ensheathing cells which were suitable for transplantation. Methods Olfactory ensheathing cells 5-month pregnant women were asolated and cultured. The ceils were stained for the detection of positive rate. Results Cultured olfactory ensheating cells （OECs） appeared with bipolar or multipolar slender processes. P75, glial fibrillary acidic protein （GFAP） immunocytochemistry was positive. The positive rate of olfactory ensheathing ceils was 95.3%. Conclusion The reported method is simple and easy, suitable for cell transplantation research.

关 键 词：嗅球 嗅鞘细胞 细胞培养 

分 类 号：R322.9[医药卫生—人体解剖和组织胚胎学]