2860例巨细胞病毒不同水平感染患者T淋巴细胞亚群免疫状态的研究  

Study of immune status of T lymphocytic cell subgroup for the patients with cytomegalovirus active infection at different levels

作　　者：李丹[1] 李力[2] 李霞[2] 闻良珍[3] 

机构地区：[1]华中科技大学同济医学院附属中西医结合医院,湖北武汉430020 [2]武汉市第一医院妇产科产前诊断中心实验室 [3]华中科技大学同济医学院附属同济医院

出　　处：《中国妇幼保健》2007年第25期3580-3582,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助(项目批号:30271673)

摘　　要：目的:从细胞及基因不同水平观察育龄妇女巨细胞病毒(HCMV)感染的细胞免疫功能变化及临床意义。方法:用RT-PCR及ELISA法检测2860例育龄妇女HCMV-IgM及HCMV-mRNA阳性率,流式细胞仪免疫荧光法检测其T淋巴细胞亚群细胞含量。SPSS11.5软件进行相应的统计分析。结果:HCMV-IgM(+)组CD4+、CD4+/CD8+、NK细胞显著降低,与HCMV-IgM(—)组比较,P<0.05;HCMVmRNA(-)组与HCMVmRNA(+)组比较,P<0.01;统计学有显著性差异。结论:病毒活动性感染时会一定程度地抑制机体的细胞免疫系统发挥正常功能,患者外周血T亚群细胞比值变化与病毒的复制活跃程度有明显关系。对指导育龄妇女感染的临床治疗、判断预后有一定意义。Objective： To observe the changes of immune status and clinical significance with cytomegalovirus （HCMV） infection at cell and gene levels. Methods： The positive rate of HCMV -IgM and HCMV -mRNA were detected by ELISA and RT - PCR method from 2860 cases to count T lymhocyte subsets by flow cytometer. The SPSS 11.5 for Win was used for statistic analysis. Results： CD4^＋ , CD4 ^＋/CD8^＋ and NK cell of group HCMV - IgM （ ＋ ） were significantly decreased compared with those in group HCMV - IgM （ - ） （P 〈 0. 05） ; CD4 ^＋ , CD4 ^＋/CD8^＋and NK cell in group HCMV mRNA （ ＋ ） were significantly lower than those of HCMV mRNA （ - ） （P 〈 0. 01 ） . Conclusion： HCMV active infection would decrease the normal function of cell immune system, the changes of T lymphocytic cell subsets are significantly related to the level of viral gene activity. There is significance to clinical therapy and prognosis of women with HCMV active infection.

关 键 词：HCMV—mRNA 免疫状态 T淋巴细胞亚群 

分 类 号：R173[医药卫生—妇幼卫生保健]