普伐他汀和阿司匹林对CD40-CD40L表达及斑块稳定性的影响  

Effects of pravastatin and aspirin on expression of CD40-CD40L and plaque stability

作　　者：陈朝婷[1] 盛净[1] 汤政德[1] 陈祥华[1] 

机构地区：[1]上海市交通大学医学院附属第九人民医院,上海200011

出　　处：《中国新药与临床杂志》2007年第8期565-569,共5页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的:研究普伐他汀和阿司匹林对兔腹主动脉粥样硬化斑块CD40-CD40L表达及斑块稳定性的影响。方法:新西兰大白兔32只予高脂饲养16wk及腹主动脉球囊损伤术建立腹主动脉粥样硬化模型,分4组:P组(普伐他汀)、A组(阿司匹林)、A+P组(联合用药)与N组(不用药),分别予相应药物灌胃8wk。通过免疫组化检测比较各组兔腹主动脉粥样硬化斑块内CD40-CD40L表达、MMP-1、胶原及脂质含量变化,超声检测腹主动脉内膜-中膜厚度(IMT)改变,ELISA法测定血清C反应蛋白(CRP)变化,分析2药干预动脉粥样硬化斑块稳定性的机制。结果:用药各组兔腹主动脉斑块内CD40-CD40L表达较N组显著受抑,普伐他汀作用更显著(P组和A+P组均P<0.01);同时各用药组MMP-1的表达、IMT增厚及CRP增高较N组均得到明显抑制(P<0.01),P组和A+P组较N组斑块内脂质显著减少(P<0.01),胶原含量明显增加(P<0.01)。结论:普伐他汀和阿司匹林都可抑制斑块CD40-CD40L系统的表达,促进斑块的稳定性。普伐他汀促进斑块稳定的机制可能是多方面的。AIM： To study the effects of pravastatin and aspirin on the expression of CD40-CD40L and plaque stability. METHODS： A total of 32 New Zealand white rabbits were randomized into 4 groups after underwent balloon-induced arterial wall injury, then were given a diet of high lipid for 16 weeks followed by a high lipid diet for 1 week to establish atherosclerotic plaque model. Eight weeks after operation, rabbits were exposed to one of four treatments： no drug （N group）, pravastatin （P group）, aspirin （A group）, combination of pravastatin and aspirin （A＋P group）. The expression of CD40-CD40L, MMP-1, content of collagen and lipid on plaque were measured by immunohistochemical staining, IMT was detected by ultrasound and the changes of serum CRP was tested with ELISA. RESULTS： The mechanism of drug intervention on plaque stability was analyzed. Compared with N group, the expression of CD40-CD40L on abdominal aorta plaque was significantly suppressed in other three groups, especially in P and A＋P groups （P 〈 0.01）. MMP-1 expression, IMT and CRP were decreased significantly （P 〈 0.01） in drug using groups. Lipid decreased and collagen increased significantly on plaque in P and A＋P groups （P 〈 0.01）. CONCLUSION： Both pravastatin and aspirin can inhibit the expression of CD40-CD40L on plaque and promote plaque stability. The mechanism of pravastatin promoting plaque stability may be multifactorial.

关 键 词：动脉硬化 抗原 CD40 CD40配体 普伐他汀 阿司匹林 斑块稳定性 

分 类 号：R972.6[医药卫生—药品]