同期输注供者骨髓间充质干细胞对肝移植大鼠免疫的影响  

Immunomodulatory effects of simultaneous injection of mesenchymal stem cells in rats with liver transplantation

作　　者：张清军[1] 高毅[1] 潘明新[1] 姚坤厚[1] 汪艳[1] 

机构地区：[1]南方医科大学珠江医院肝胆外科,广州510282

出　　处：《广东医学》2007年第9期1401-1403,共3页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:C03020504)

摘　　要：目的探讨肝移植同时输注供者骨髓间充质干细胞对受体肝移植免疫的影响。方法实验分为空白对照组(A组)、移植组(B组)和输注供者骨髓间充质干细胞组(C组)。A组SD大鼠5只,只行剖腹探查,B,C组Wistar和SD大鼠各10只。行Wistar-SD大鼠肝移植同时,C组提取供体骨髓间质干细胞同期输注给受者,B组给予输注生理盐水。观察术后第7天肝功能的变化、病理改变和受体生存期。结果肝移植同时输注供者骨髓间充质干细胞,ALT,AST,TBIL,ALB较B组显著降低,生存期明显延长,病理改变仅呈急性轻度排斥反应,而单纯移植组呈急性重度排斥反应。结论肝移植同时输注骨髓间充质干细胞促进受体对移植肝的免疫耐受。Objective To study the immunomodulatory effects of simultaneous injection of mesenchymal stem cells in rats with liver transplantation. Methods Three groups of rats were used in this study： the first group （ Group A） consisting of 5 SD rats, other group consisting of 10 SD rats and 10 Wistar rats with the former as the recipients and the latter as the donors for liver transplantation. The first group only had laparotomy. The second group （ Group B） received orthotopic liver transplantation. In the third group （ Group C ）, the recipient SD rats received simultaneous injection of mesenchymal stem cells of the donor Wistar rats and liver transplantation. The blood levels of ALT, AST, ALB and TBil were determined and liver pathological changes and survival was observed. Results The blood levels of ALT, AST, ALB and TBil were significantly lower, survival was significantly longer and pathological changes was milder in the rats receiving infusion of mesenchymal stem cells from donors 1 week before the liver transplantation than those in Group B （ P 〈 0. 05 ）. Conclusion Simultaneous injection of mesenchymal stem cells from donors can induce immune tolerance in rat liver transplantation.

关 键 词：骨髓间充质干细胞 肝移植 免疫耐受 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]