通心络对小鼠病毒性心肌炎抗心肌纤维化作用  

Anti-Fibrosis Effect of Tongxinluo on Cardiac Muscle of Rats with Chronic Viral Myocarditis

作　　者：忻尚平[1] 程志清[1] 

机构地区：[1]浙江医院心脏康复科,杭州310013

出　　处：《浙江中西医结合杂志》2007年第9期537-538,共2页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药管理局课题No:2006C011

摘　　要：目的:观察通心络胶囊抗小鼠病毒性心肌炎慢性期心肌纤维化作用,并探讨其抗心肌纤维化的作用机制。方法:小鼠腹腔注射柯萨奇B3病毒,建立病毒性心肌炎慢性期心肌纤维化小鼠模型。随机分模型对照组、通心络胶囊低、中、高剂量组和正常对照组。用药4周后处死动物,通过免疫组化染色观察致纤维化细胞因子—转化生长因子(TGF)和血小板衍生生长因子(PDGF)的定量表达。结果:免疫组化染色示,正常组TGF、PDGF呈低水平表达,模型组较正常组表达水平显著升高(P<0.01),通心络各剂量组的TGF、PDGF表达水平均有不同程度降低。其中高剂量组和模型对照组比较,差异有显著性意义(P<0.05)。结论:通心络胶囊各剂量组均有抗心肌纤维化作用,其中以高剂量组效果最为明显。其作用机制可能与抑制TGF-β1、PDGF-BB的表达有关。Objective： To obverse the anti-fibrosis effect and mechanism of Tongxinluo on cardiac muscle of rats with chronic viral myocarditis （VMC）. Methods：Rats with chronic VMC were established by injecting CVB3 into abdominal cavity and randomly divided into the model contrast group, the tongxinluo group with low dose, the tongxinluo group with middle dose, the tongxingluo group with high dose, and the normal contrast group. The quantity express of TGF and PDGF was observed by immunohistochemical coloration techniques after 4 weeks treatment. Results：The quantity express of TGF and PDGF in the normal contrast group was in low level. The quantity express level of TGF and PDGF in the model contrast group was obviously improved than that in the normal contrast group （ P 〈 0.01 ）. The quantity express level of TGF and PDGF in the tongxinluo group with different dose was decreased in different degree. The difference in the group with high dose and low dose had significant meaning （P 〈 0.05 ）. Conclusion： The tongxinluo group with different dose has anti-fibrosis effect on cardiac muscle of rats with chronic VMC. Among them, the group with high dose has the best effect. The experiment show the mechanism has relationship with the express inhibition of TGF-β1 and PDGF-BB.

关 键 词：小鼠 病毒性心肌炎 通心络胶囊 胶原 细胞因子 

分 类 号：R285.5[医药卫生—中药学]