偶氮氯膦Ⅲ-KIO_4体系阻抑反应动力学光度法测定蛋白质  

Inhibitive-kinetic spectrophotometric determination of protein based on reaction between potassium periodate and Chlorophosphonazo Ⅲ

作　　者：孙晓艳[1] 李建平[1] 李天德[1] 

机构地区：[1]桂林工学院材料与化学工程系,桂林541004

出　　处：《分析试验室》2007年第8期37-40,共4页Chinese Journal of Analysis Laboratory

基　　金：广西教育厅科研(200508135)项目资助

摘　　要：基于KIO4在H2SO4介质和沸水浴中可氧化偶氮氯膦Ⅲ褪色，而蛋白质能灵敏地阻抑该氧化褪色反应，建立了测定痕量蛋白质的新方法。蛋白质测定范围为0-6μg/mL，方法的检出限为2.9×10^-10g/L，回收率为96%-109%。测定了动力学参数，并对反应机理做了研究。该方法可用于实际样品的测定。A new method for the detennination of trace bovine serum albumin （BSA） was proposed. It was based on the inhibitory effect of protein on discoloring reaction between KIO4 and chlomphosphonazo Ⅲ in dilute stulfuric acid solution at 100℃. The calibration graph is rectilinear from 0 to 6 μg/mL for BSA and the detection limit is 2.9 × 10^-10 g/L. Kinetic parameters and reaction mechanism have been studied. This method is simple and sensitive. It has been applied satisfactorily to the detennination of BSA in milk samples.

关 键 词：动力学分光光度法 蛋白质 阻抑反应 

分 类 号：O657.32[理学—分析化学]