依文思蓝光度法测定阿昔洛韦及其分析应用  

Spectrophotometric method for determination of aciclovir with evans blue and its analytical application

作　　者：秦宗会[1] 付维权 汪正文 谭蓉[4] 

机构地区：[1]涪陵师范学院化学及环境科学系,涪陵408003 [2]重庆涪陵第五中学,涪陵408000 [3]重庆武隆中学校,武隆408500 [4]太极集团涪陵制药厂,涪陵408000

出　　处：《分析试验室》2007年第8期69-72,共4页Chinese Journal of Analysis Laboratory

基　　金：重庆市第二批中青年骨干教师资助计划;涪陵师范学院科研(flsy2006-25)项目资助

摘　　要：在pH 5.74 HAc-NaAc缓冲介质中，阿昔洛韦（ACV）与依文思蓝（EB）反应形成离子缔合物，溶液颜色发生明显改变，最大褪色波长为638 nm。在此波长处，阿昔洛韦的浓度与褪色程度呈良好线性关系，从而建立测定阿昔洛韦的光度法。在最大褪色波长处，阿昔洛韦的浓度在0-2.01×10^-5mol/L范围内遵守比尔定律，表观摩尔吸光系数1.71×10^4L·mol^-1·cm^-1，检出限为7.47×10^-7mol/L。方法具有较高的灵敏度和良好的选择性，可用于实际药品、血浆及尿液中阿昔洛韦的测定。In HAc-NaAc pH 5.74 buffer medium, aciclovir （ACV） reacts with Evans Blue （EB） to form an ion-association complex, the colour of the solution changes obviously with the maximum fading color wavelength at 638 nm. The fading colour intensity is also proportional to the concentration of aciclovir, a sensitive spectrophotometric method is developed for the determination of aciclovir. The concentration of aciclovir in the range of 0 - 2.01 × 10^-5 mol/L obeys Beer＇s law at the maximum fading color wavelength, the apparent molar absorptivity is 1.71×10^4 L/（mol· cm）, and the detection limit is 7.47 × 10^-7 mol/L. The method has high sensitivity and fairly good selectivity, the method is applied to the determination of aciclovir in medicine samples and human serum and human urine with satisfactory resuits.

关 键 词：分光光度法 依文思蓝 阿昔洛韦 

分 类 号：O657.32[理学—分析化学]