等色吸收荧光猝灭法快速测定食品中苏丹红Ⅰ  

A quick determination of Sudan Ⅰ in food by fluorescence quenching-isochromatic absorption

作　　者：刘保生[1] 王云科[1] 曹冬林[2] 傅丽[3] 

机构地区：[1]河北大学理化分析中心河北省分析科学技术重点实验室,保定071002 [2]河北工程大学,邯郸056038 [3]廊坊师范学院化学系,廊坊065000

出　　处：《分析试验室》2007年第8期87-90,共4页Chinese Journal of Analysis Laboratory

摘　　要：在乙醇介质中，强荧光染料吖啶的荧光波长478 nm与苏丹红Ⅰ的吸收光波长480 nm十分接近，由于等色性苏丹红Ⅰ能够有效吸收吖啶发出的荧光，使吖啶在激发波长λex=410 nm、荧光波长λem=478 nm处荧光猝灭，依此建立了荧光猝灭测定苏丹红Ⅰ的新方法。该方法快速、简便，其线性范围为3.72-37.2 mg/L，方法检出限为1.1 mg/L。用于辣椒、辣椒酱食品中痕量苏丹红Ⅰ的测定。6次平行测定，相对标准偏差为0.8%-2.7%，加标回收率为89.5%-104.0%。Depending on the isochromatism between the fluorescence of acridine and the absorption of Sudan Ⅰ , at λex/λem = 410/478 mm, Sudan Ⅰ absorbed the fluorescence of acridine effectively in the medium of ethanol, which made the fluorescence of acridine quenched, so a new method for the determination of Sudan Ⅰ was established. The linear range of Sudan Ⅰ was 3.72 - 37.2 mg/L. The measurement has been applied to the determination of Sudan in capsicum powder and chili sauce. The relative standard deviation were 0.8 % - 2.7 % for 6 parallel determinations of Sudan Ⅰsamples and the recoveries of Sudan Ⅰ were 89.5% - 104.0%. The detection limit of the method was up to 1.1 mg/L.

关 键 词：荧光猝灭 苏丹红Ⅰ 吖啶 

分 类 号：O657.31[理学—分析化学]