长期服用低剂量硝酸镧对大鼠血清胰岛素及肝细胞糖原的影响  

Effect of a long-term administering of low dosage La(NO_3)_3 on insulin in serum and hepatocyte glycogen in rat

作　　者：黄可欣[1] 马洪喜[2] 石博[1] 李树蕾[3] 崔丽[1] 

机构地区：[1]吉林大学基础医学院形态学中心,吉林长春130021 [2]吉林大学第一医院病理科,吉林长春130021 [3]吉林大学基础医学院组胚教研室,吉林长春130021

出　　处：《军医进修学院学报》2007年第4期249-251,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金重大课题资助[29890280-3(2)]

摘　　要：目的:观察低剂量硝酸镧经长期口服后对大鼠血清胰岛素、血糖及肝细胞糖原的影响。方法:以Wistar大鼠为对象,每日经口给予硝酸镧(10.0 mg/kg、2.0 mg/kg、0.2 mg/kg、0.1 mg/kg)6和7个月后,利用放射免疫分析技术和显微图像分析技术检测了大鼠血清中胰岛素的水平、血糖水平及肝细胞糖原的水平。结果:给予硝酸镧6个月后,10.0 mg/kg组雌性、雄性大鼠胰岛素水平下降、血糖水平升高及肝细胞糖原的灰度值升高与对照组有显著性差异;0.1 mg/kg、0.2 mg/kg、2.0 mg/kg组雄性大鼠胰岛素水平升高、血糖水平下降、肝细胞糖原的灰度值下降且0.2 mg/kg组与对照组有显著性差异;0.1 mg/kg、0.2 mg/kg、2.0 mg/kg组雌性大鼠胰岛素水平、血糖水平、肝细胞糖原的灰度值与对照组无显著性差异。灌胃6个月后,停药1个月检测,雌性、雄性大鼠各组胰岛素水平、血糖水平、肝细胞糖原的灰度值基本恢复正常。结论:低剂量硝酸镧长期作用可以引起大鼠血清中胰岛素水平、血糖水平、肝细胞糖原发生变化,这种变化可在短期内恢复正常;较低浓度的硝酸镧对胰岛素、血糖和肝细胞糖原有促进作用。Objective：To investigate the long-term effects of low dosage La（NO3）3 on insulin and blood glucose in serum and hepatocyte glycogen in rat. Methods： Levels of Insulin and blood glucose and hepatocyte glycogen in rat were measured by radiommunoassay and microscopy image analysis system after administrated with La（NO3）3 （ 10. 0 mg/kg, 2. 0 mg/kg, 0.2 mg/kg, 0. 1 mg/kg） for six and seven months. Results： Having taken La（NO3）3 for 6 months, insulin levels in the 10.0 mg/kg group were reduced, blood glucose and hepatocyte glycogen levels in the 10.0 mg/kg group were increased, both in the sera of female and male rats; insulin levels, blood glucose levels and hepatocyte glycogen levels in the 0. 1 mg/kg, 0.2 mg/kg, 2.0mg/kg group, low dose treated groups was increased and later was reduced in the sera of the male. After fed by normal chow without La（NO3）3 for one month, the levels of insulin, blood glucose and hepatocyte glycogen returned to normal again. Conclusion：A long-term low dosage La（NO3）3 could change insulin、 blood glucose and hepatocyte glycogen levels. Insulin, blood glucose and hepatocyte glycogen levels could back to normal levels after a short time of feeding without La. La（NO3）3. In concentration of 0.2 mg/kg and 0.1 mg/kg, it had the capacity to increase the levels of insulin, blood glucose and hepatocyte glycogen.

关 键 词：金属稀土 胰岛素 肝糖原 大鼠 血糖 

分 类 号：R348.5[医药卫生—基础医学]