钾通道开放剂对抗原诱发大鼠肥大细胞释放组胺的抑制作用  

INHIBITION OF ALLERGIC HISTAMINE RELEASE FROM RAT PERITONEAL MAST CELLS BY POTASSIUM CHANNEL OPENERS

作　　者：陈志刚[1] 徐建华[1] 

机构地区：[1]浙江医科大学药学系药理学教研室

出　　处：《浙江医科大学学报》1997年第2期49-51,共3页

基　　金：国家自然科学基金!39270788

摘　　要：本文报道钾通道开放剂米诺地尔（Min）、二氮嗪（Dia）和钙拮抗剂硝苯啶（Nif）对抗原诱发大鼠腹腔肥大细胞（PMC）释放组胺的影响。以卵清蛋白免疫大鼠并触发PMC释放组胺。给大鼠Min200mg／kg灌胃；用含有Dia的孵化液预处理；或用Nif125mg／kg灌胃后，触发剂诱发PMC的组胺释放率与对照组比较均有显著差异（P＜0.01）。结果提示，肥大细胞可能存在钾通道，钾通道开放剂可能具有治疗过敏性疾病的前景。The effects of potassium channel openers minoxidil (Min), diazoxide (Dia) and calcium antagonist nefedipine (Nif) on allergic histamine release from rat peritoneal mast cells (PMC) were investigated. Male SD rats were sensitized with 50μg of ovalbumin and 10 mg of Al(OH)3 ip.Two weeks later, PMC suspensions were prepared and 10μg/ml ovalbumin added to the suspensions as antigen. The results showed that the percentage of histamine release in control was 42. 7 ±9. 0%. After treatment with Min (200 mg/kg ig), Dia (500μmmol/L in vitro )and Nif (125mg/kg ig ) the percentages of histamine release were 18. 1±9. 7%, 19. 5±3. 6% and 11. 7±4. 7% respectively. In comparison with the control the differences were statistically significant (P<0. 01 ). The results suggest that calcium channels and potassium channels may by involved in the allergic histamine release from rat PMC. Potassium channel openers may be a group of useful agents for the treatment of allergic diseases.

关 键 词：钾通道开放剂 米诺地尔 二氮嗪 肥大细胞 过敏 

分 类 号：R972[医药卫生—药品] R976[医药卫生—药学]