福建省青枯雷尔氏菌脂肪酸多态性研究  

Polymorphism of Fatty Acid of Ralstonia solanacearum in Fujian Province

作　　者：王秋红[1,2] 陈亮[1] 林营志[3] 朱育菁[3] 蓝江林[3] 杨淑佳[1,2] 刘波[3] 

机构地区：[1]厦门大学生命科学学院 [2]福建省农业科学院生物技术研究所,福州350003 [3]福建省农业科学院生物技术研究所

出　　处：《中国农业科学》2007年第8期1675-1687,共13页Scientia Agricultura Sinica

基　　金：国家"863"项目(2002AA244031);福建省青年科技人才创新项目(2003.J046)

摘　　要：【目的】利用气相色谱技术检测福建省的40株青枯雷尔氏菌(Ralstonia solanacearum)菌株细胞内的脂肪酸,分析其脂肪酸分布的多态性;研究青枯雷尔氏菌脂肪酸多态性与青枯雷尔氏菌现有种下分化方法之间的关系。【方法】对40株青枯雷尔氏菌脂肪酸进行气相色谱分析,比较同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌脂肪酸的分布;对40株青枯雷尔氏菌脂肪酸进行聚类分析,分析聚成的各类青枯雷尔氏菌脂肪酸的特点以及脂肪酸多态性与其生理小种、生化型和致病性之间的关系。【结果】同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌,其脂肪酸都存在着明显的多态性;对40株青枯雷尔氏菌的脂肪酸进行聚类分析,可以聚成3类,即groupⅠ、groupⅡ和groupⅢ;青枯雷尔氏菌生理小种1存在着不同的脂肪酸类群,青枯雷尔氏菌脂肪酸多态性与其生化型之间不存在相关性,但是脂肪酸和致病性之间存在一定的相关性:groupⅠ为无致病性菌株,groupⅡ为过渡性菌株,groupⅢ为强致病性菌株。【结论】福建省青枯雷尔氏菌脂肪酸分布存在着明显的多态性;青枯雷尔氏菌脂肪酸多态性与致病性之间存在一定的相关性,脂肪酸有望成为青枯雷尔氏菌小种鉴定的新指标。[Objective] The fatty acids of 40 strains of Ralstonia solanacearum isolated from different hosts in the fields in Fujian Province were detected by gas chromatography （GC）. The polymorphism of R. solanacearum fatty acids relating to the pathogenicity was observed. [Method] The MID! system and cluster analysis were introduced in analyzing fatty acids to display the relations among the polymorphism, race, biovartype and pathogenicity. [Result] The results showed that the patterns of fatty acids were significant different in R. solanacearum strains both isolated from the different hosts and the different body parts of the same hosts. According to the fatty acids the strains were clusteréd into three groups, e.g. group I relating to the strains with non-pathogenicity, group II in which the strain pathogenicity was changeable with some virulent and avirulent ones, and group III respondent to high pathogenicity. It was proved that the model of fatty acids has no relations to races and biovartypes in R. solanacearum. [ Conclusion ] It is the fist time to describe the polymorphism of fatty acids in R. solanacearum in this paper. The pathogenicity could be grouped by the models of fatty acids to distinguish the pathogenicity, which could be used in the identification ofR. solanacearum under species differentiation.

关 键 词：青枯雷尔氏菌 脂肪酸 多态性 气相色谱 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]