利用CAPS标记转育野生番茄的高产基因  

Introgression of High Yield Genes from Lycopersicon.hirsutum acc.LA1777 Using CAPS Marker

作　　者：李红[1,2] 杜永臣[1] 王孝宣[1] 宋明[3] 高建昌[1] 国艳梅[1] 朱德蔚[1] 戴善书[1] 

机构地区：[1]西南大学园艺园林学院 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]中国农业科学院蔬菜花卉研究所

出　　处：《中国农业科学》2007年第8期1738-1745,共8页Scientia Agricultura Sinica

基　　金：北京市自然科学基金资助项目(6042023);农业部蔬菜遗传与生理重点开放实验室资助项目

摘　　要：【目的】将野生多毛番茄中的高产基因渗入到普通番茄骨干亲本以创造高产番茄育种材料。【方法】TA1229是野生多毛番茄(Lycopersicon hirsutum acc.)LA1777的一个近等基因系,带有来源于LA1777的高产基因(QTL)。通过杂交、回交和分子标记辅助选择方法对TA1229×9706的BC1群体进行遗传分析。【结果】获得23个比TA1229含有的外源染色体片段更短的渐渗系,其中16个渐渗系为高产番茄材料,并检测到了一个影响番茄成熟果实平均重量的QTL,将其定位于标记TG53和TG158之间。【结论】16份渐参系可用作为高产番茄新种质。[ Objective ] Using high yield genes from wild tomato can enrich tomato breeding resources and accelerate tomato breeding programs. [Method] In this study, the near-isogenic line TA1229, containing a 24-cM introgression at the bottom of chromosome 1 from Lycopersicon hirsutum acc.LA1777, affected several high yield traits. The TA 1229x9706 BC 1 population was analyzed by marker-assisted selection and the traits of the population were evaluated. [ Result ] Twenty-three recombinant individuals carrying a shorter segment than TA1229 were obtained. Among them,16 lines with the chromosome 1 recombinant segment increased tomato yield. QTL affecting yield was found between TG53 and TG 158. [ Conclusion ] Sixteen recombinant lines are useful for improvement of tomato varieties.

关 键 词：CAPS QTL 分子标记辅助选择 高产 番茄 

分 类 号：S641.2[农业科学—蔬菜学]