东亚及南亚固有绵羊群体的遗传结构研究  

Genetic Structure of Native Sheep Populations in East and South Asia

作　　者：耿荣庆[1,2] 常洪[1] 王兰萍[3] 杨章平[1] 孙伟[1] 冀德君[1] 角田健司 

机构地区：[1]扬州大学动物科学与技术学院 [2]盐城师范学院生命科学与技术学院,盐城224002 [3]盐城师范学院生命科学与技术学院 [4]昭和大学医学部

出　　处：《中国农业科学》2007年第8期1786-1794,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金;国际合作项目(3057132330213001;30210103007;3041013150);江苏省高校自然科学基础研究项目(06KJD230203)

摘　　要：【目的】分析东亚及南亚固有绵羊群体的遗传结构。【方法】以多座位电泳法检测中国4个绵羊群体结构基因座上的变异,同时引用11个绵羊群体的同类资料作为参考。【结果】15个群体结构基因座平均杂合度和有效等位基因数分别为0.2746和1.559;平均杂合度和有效等位基因数都是蒙古国绵羊群体最大,蒙古国、中国、越南、孟加拉国和尼泊尔绵羊群体的遗传多样性依次减小。绵羊群体间遗传分化系数在0.0126～0.3083之间,平均为0.148,说明遗传变异主要存在于群体内,占总变异的85.2%。群体地理位置的远近与遗传距离间无相关性;大多数群体间基因流通畅,使得群体间地理位置的远近与遗传距离不完全一致。东亚及南亚15个固有绵羊群体大致可分为两大类群:一类包括了中国和蒙古国的部分群体,另一类则包括了中国云南绵羊、尼泊尔以及孟加拉国的部分群体,其余群体逐步汇入这两大类群。【结论】东亚及南亚15个固有绵羊群体间的遗传分化程度相对不高;地理隔离不是影响群体间遗传分化的主要原因;亲缘关系聚类分析可将15个绵羊群体大致分为两大类群。[Objective] This study was designed to analyze the genetic structure of the native sheep populations in East and South Asia. [Method] Variations of structural loci among 4 sheep populations in China were examined by the method of multiloci electrophoresis,and similar data from 11 sheep populations were quoted as basic references. [Result] The average heterozygosity and effective number of alleles among 15 populations were 0.2746 and 1.559 respectively. Mongolian sheep possessed the largest average heterozygosity and effective number of alleles. Genetic diversity of sheep populations in Mongolia, China, Vietnam, Bangladesh and Nepal reduced in turn. Coefficients of the genetic differentiation were between 0.0126 and 0.3083, with the average of 0.148 ,demonstrating that genetic variations lied mainly in populations, with 85.2 % of the total variations. There existed no correlation between geographical distances and genetic distances. Gene flow was smooth between most populations, which led to inconsistency between geographical distances and genetic distances. Fifteen native sheep populations in East and South Asia could be divided into two groups,one group including part populations of China and Mongolia,another including Yunnan populations of China,part populations of Nepale and Bangladeshi. Other populations did not cluster together and went into the above-mentioned two groups. [Conclusion]Genetic differentiation of 15 native sheep populations in East and South Asia was relatively low. Geographical isolation was not the main reason affecting genetic differentiation. Fifteen sheep populations could be divided into two groups according to the phylogenetic relationships.

关 键 词：绵羊 遗传结构 基因流 遗传分化 

分 类 号：S826[农业科学—畜牧学]