他莫昔芬对肾间质纤维化影响的实验研究  

Effect of Tamoxifen on interstitial fibrosis in UUO rats

作　　者：姚刚[1] 许宏岳[1] 杨春[1] 吴限[1] 徐玲玲[1] 

机构地区：[1]南京医科大学第二附属医院肾脏科,江苏南京210011

出　　处：《医学研究生学报》2007年第8期816-818,共3页Journal of Medical Postgraduates

基　　金：江苏省教育厅基金资助项目(批准号:03KJD320125)

摘　　要：目的:探讨他莫昔芬对肾组织纤维化的影响及作用机制。方法:雌性SD大鼠,建立单侧输尿管梗阻模型(UUO),设置3组:①模型组(n=6),行左侧输尿管结扎术,0.3m l无菌等渗盐水灌胃,每天2次;②治疗组(n=6),他莫昔芬5mg/kg(0.3m l无菌等渗盐水配置)灌胃,每天2次;③假手术组(n=6)。术后第9 d处死各组大鼠。采用光镜和免疫组化观察肾间质纤维化、炎细胞浸润和α平滑肌肌动蛋白(α-SMA)表达的变化。结果:①治疗组大鼠肾间质纤维化程度较模型组明显减轻。②模型组第9 d时大鼠肾组织α-SMA表达明显增强,采用他莫昔芬治疗后,肾组织α-SMA表达的异常增强能得到有效抑制,肾组织炎细胞浸润明显减少。结论:昔芬类药物具有减轻肾间质纤维化的作用,而这一作用与其能有效阻抑肾组织上皮细胞-间充质细胞转分化和减少肾间质炎细胞浸润有关。Objective ：To investigate the effects of Tamoxifen on renal interstitial fibrosis in rat. Methods ：Female SD rats were divided into 3 groups randomly： （1）UUO group（6 rats）, （2）treatment group （6 rats） ： UUO ＋ Tamoxifen per kg lOmg/day by gastrogavage, （3）sham operation group（6 rats）. Kidneys were harvested at day 9 after UUO. The renal tissues were examined by HE, PAS and Masson. Tissue α-SMA protein expression was determined by Immunohistochemistry. Results：UUO at 9 days significantly activated α-SMA protein expression, induced interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of UUO group compared with sham operation group. Conversely daily administration of Tamoxifen significantly inhibited α-SMA expression and decreased interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of treatment group. Conclusion：Tamoxifen had beneficial effects on interstitial fibrosis in SD rats with UUO, and this effect may be associated with Tamoxifen inhibits α-SMA expression and inflammatory cell infiltration.

关 键 词：肾脏 纤维化 他莫昔芬 

分 类 号：R692[医药卫生—泌尿科学]