Bid蛋白在瘢痕疙瘩中的高表达及其意义  

Over Expression and Its Significance of Bid Protein in Keloid

作　　者：聂芳菲[1] 陈东明[1] 赵霞[1] 孙东杰[1] 

机构地区：[1]北京大学第三医院成形外科,北京100083

出　　处：《中国微创外科杂志》2007年第8期812-814,共3页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金资助项目(编号:30070778)

摘　　要：目的检测促凋亡分子Bid蛋白在瘢痕疙瘩中的表达,研究其与瘢痕疙瘩发生的关系。方法用免疫组织化学法和免疫印迹法(western blotting)分别检测12例瘢痕疙瘩与12例正常皮肤组织中Bid蛋白的表达,定量分析Bid蛋白在瘢痕疙瘩中的表达。结果①Bid蛋白在瘢痕疙瘩和正常皮肤组织的角质细胞、成纤维细胞、炎症细胞以及血管内皮细胞、毛囊、汗腺、皮脂腺等细胞的胞质及部分胞核中表达。②表皮中Bid蛋白表达量两种组织差异无显著性(P>0.05);凋亡启动的细胞数量正常皮肤(1738.33±348.89/mm2)高于瘢痕疙瘩(891.67±395.00/mm2)(t=-5.565,P=0.000)。在真皮中的Bid蛋白阳性成纤维细胞数,瘢痕疙瘩(911.67±323.61/mm2)高于正常皮肤(220.00±80.00/mm2)(t=7.188,P=0.000)。免疫印迹法实验结果进一步证实Bid蛋白含量瘢痕疙瘩(0.46±0.08)高于正常皮肤(0.02±0.01)(t=18.905,P=0.000)。结论①正常皮肤表皮凋亡启动的细胞多于瘢痕疙瘩;②Bid蛋白在瘢痕疙瘩真皮组织中表达增高。Objective To detect the expression of pro-apoptosis Bid protein in keloid, and to investigate the relationship between Bid protein and development of keloid. Methods lmmunohistochemistry and western blotting were used to detect the expression of Bid protein in 12 cases with keloid and 12 cases with normal skin tissue. The expression was analyzed quantitatively. Results （1）Bid was expressed in plasma and/or nucleus of keratinocytes, fibroblasts, inflammatory cells and endothelial cells, as well as cells in hair follicles, sweat glands and sebaceous glands in both keloid and normal skin tissues. （2）Although the expression levels of Bid in epidermis were not significantly different between the two groups （P 〉 0.05） , there were more apoptosis-starting cells found in normal skin （ 1738.33 ±348.89/mm^2 ） than in keloid （891.67 ± 395.00/mm^2 ） （ t = - 5. 565, P = 0. 000）. The number of positive stained fibroblast cells in dermis significantly increased in keloid group （911.67 ± 323.61/mm^2） , compared with that in normal skin group （220.00 ± 80.00/mm^2） （t = 7. 188, P = 0. 000）. This result that the protein expression level of Bid was higher in keloid （0.46±0.08） than that in normal skin tissue （0.02 ±0.01） （t = 18. 905, P =0.000） was proven by western blotting test. Conclusions The number of apoptosis-starting cells in epidermis of normal skin is more than that in keloid. Bid protein in dermis of keloid is over expressed.

关 键 词：Bid蛋白 瘢痕疙瘩 检测 

分 类 号：R622[医药卫生—整形外科]