正常孕妇、新生儿及异常胎儿血清中人微小病毒B19感染的研究  

Human Parvovirus B19 Infection in Perinatal Transmission and Abnormal Fetuses

作　　者：钟梅[1] 曹虹[1] 温淑娟[1] 陈翠华[1] 张绍云[1] 周凤琴[1] 王香云 

机构地区：[1]第一军医大学南方医院妇产科

出　　处：《中华妇产科杂志》1997年第4期205-207,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：广东省自然科学基金

摘　　要：目的：了解人微小病毒Ｂ１９在母婴中的感染状况以及该病毒对胎儿的危害。方法：应用聚合酶链反应技术（ＰＣＲ）检测３５０例正常孕妇及新生儿血清、９例异常胎儿组织血清人微小病毒Ｂ１９ＤＮＡ。结果：正常孕妇血清Ｂ１９ＤＮＡ阳性率为１．１４％，新生儿脐血阳性率为０．２８％；９例异常胎儿中有６例孕妇血清及脐血Ｂ１９ＤＮＡ阳性（６／９）。应用原位杂交的方法在ＰＣＲ阳性的异常胎儿脑和脾组织中也检出了Ｂ１９ＤＮＡ阳性颗粒。结论：应用ＰＣＲ法检查孕妇及新生儿Ｂ１９病毒具有高度敏感、简便、特异的特点，而原位杂交方法可对病毒感染进行定位，以了解感染部位的病理变化。Objective: To investigate the perinatal infection of human parvovirus B19 in pregnant women and their fetuses. Methods: 350 sera of normal pregnant women and 9 sera of abnormal fetuses were in vestigated by polymerase chain reaction (PCR) to check the parvovirus B19 DNA. Results: The prevalence of parvovirus B19 DNA was 1.14% and 0.28% in the sera of normal pregnant women and umbilical cords, respectively. Parvovirus B19 DNA was detected in 6 sera of pregnant women and umbilical cords from 9 abnormal fetuses parvovirus DNA could be detected by in situ hybridization in the nuclei of immature hematopoetic cells within fetal brain or spleen whose PCR tests were positive. Conclusion: The results suggest that parvovirus B19 infection do exist in pregnant women and their neoantes as well as fetuses in our country. PCR and in situ hybridization could provide more sensitive, more accurate and specific methods for clinical pathologic diagnosis and epidemic investigation.

关 键 词：细小病毒B19 感染 孕妇 新生儿 胎儿 PCR 

分 类 号：R446.5[医药卫生—诊断学] R373.9[医药卫生—临床医学]