阿片μ受体在猫脊髓内的分布  

DISTRIBUTION OF μ OPIOID RECEPTOR IN THE SPINAL CORD OF THE CAT

作　　者：龚良维[1] 丁玉强[1] 吕岩[1] 郑恒兴[1] 秦秉志[1] 李继硕[1] 

机构地区：[1]第四军医大学解剖学教研室

出　　处：《解剖学报》1997年第2期147-150,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：应用免疫组织化学方法（ＡＢＣ法）观察了阿片μ受体（ｍｕ－ｏｐｉｏｉｄｒｅｃｅｐｔｏｒ，ＭＯＲ）在猫脊髓和脊神经节内的分布。在脊髓内，ＭＯＲ样免疫反应产物主要分布于神经毯（ｎｅｕｒｏｐｉｌ）内，偶尔可见阳性胞体。致密的ＭＯＲ样免疫反应产物主要分布于背角的Ⅰ、ⅠⅠ层。在胸腰髓和骶髓的中间带外侧核、中央管周围灰质、骶髓节段的后连合核和Ｏｎｕｆ核内发现有中等密度的ＭＯＲ样免疫反应产物。背索外侧部的Ｌｉｓｓａｕｅｒ束内的纤维亦呈现中等密度的免疫阳性染色。此外，脊神经节内分布大量ＭＯＲ样免疫反应细胞。切断后根后，发现术侧背角浅层内ＭＯＲ样免疫反应产物密度有明显降低；而半横断脊髓后，切断平面上下脊髓背角浅层内ＭＯＲ样免疫反应产物密度无明显改变。上述结果表明，阿片μ受体在猫脊髓内主要分布于背角浅层，终止于背角浅层的外周传入纤维中含有ＭＯＲ。Using immunohistochemical method, we investigated the distribution of μ opioid receptor (MOR) in the cat spinal cord and dorsal root ganglia. It was found that MOR like immunoreactivity (MOR LI) was mainly located in neuropil and occasionally in cell bodies of the spinal cord. Dense MOR LI was observed in the superficial laminae( Ⅰ and ⅠⅠ) of the dorsal horn. Moderate or weak MOR LI was found in the intermediolateral nucleus, gray matter encircling the central canal (lamina X), dorsal commissural nucleus and Onuf′s nucleus of the sacral segments. Moderate MOR LI was also seen within the Lissauer′s tract. In addition, many neurons with MOR LI were distributed in the dorsal root ganglia. After unilateral dorsal rhizotomy (L 6 S 3), density of MOR LI in the superficial laminae ipsilateral to the dorsal rhizotomy was markedly reduced. After semisection at the L 2 segment, however, the density of MOR LI in the superficial laminae of the dorsal horn below and above the L 2 segment showed no obvious changes. It is concluded that MOR is mainly distributed in the superficial laminae of the cat spinal cord, and some of primary afferent fibers terminating in the superficial laminae of the dorsal horn are provided with MOR.

关 键 词：阿片Μ受体 吗啡 脊髓 免疫组织化学 镇痛药 

分 类 号：R971.2[医药卫生—药品] R338.21[医药卫生—药学]