魏氏拟尾柱虫腹皮层纤毛器微管胞器的形态及形态发生  

Morphology and morphogenesis of the ciliature microtubular organelles in the ventral cortex of Paraurostyla weissei(Hyportrichida,Ciliophora).

作　　者：娄慧玲[1] 高巍[1] 倪兵[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《动物学报》2007年第4期742-749,共8页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目(No.30470201)~~

摘　　要：应用荧光紫杉醇直接荧光标记和抗α-微管蛋白抗体免疫荧光标记显示,魏氏拟尾柱虫(Paraurostyla weissei)腹面皮层纤毛器微管胞器由口围带、波动膜、额腹横棘毛和左右缘棘毛等纤毛器微管、纤毛器基部附属微管等组成。其中口围带基部微管包括小膜托架、小膜附属微管;额腹横棘毛和左右缘棘毛基部附属微管包括前纵微管束、后纵微管束和横微管束,它们由各自的纤毛器基部向皮层细胞质不同方向发射,形成腹皮层表面下微管网。结果表明,魏氏拟尾柱虫的纤毛器骨架、纤毛器附属结构也是一类以微管蛋白为基本成分的微管胞器,其中缘棘毛基部附属微管具有不同于其他纤毛虫(例如棘尾虫)中所观察到的同种微管胞器的建构特征。形态发生中,前仔虫口围带在老结构位置形成,其结构建成与部分老口围带的更新有关;老缘棘毛的结构物质对新的左、右缘棘毛的发生可能具有定位作用及物质贡献,但此后新的左、右缘棘毛列分别在老缘棘毛的右侧形成,而并非是在老缘棘毛位置分化的。在有些细胞中,新的左缘棘毛左侧另有一列棘毛,这可能是形态发生中老的左缘棘毛退化不完全产生的。The ciliature microtubular organelles in the ventral cortex of Paraurostyla weissei were analyzed with fluorescence labeling of FLUTAX and anti-a tubulin antibody. These organelles consisted of adoral zone of membranelles （AZM）, undulating membranes （UM）, frontal-ventral-transverse cirri （FVTC）, left ＆ right marginal cirri （L-＆ RMC） and the base-associated microtubules of these ciliatures. The microtubular cytoskeleton of AZM comprised membranelle brackets and its associated microtubules; the base-associated microtubules of FVTC, L-＆ RMC contained anterior longitudinal microtubules （AIM）, posterior longitudinal microtubules （PLM） and transverse microtubules （TM）, these microtubules sent forth from the ciliatures and extended to different orientation, forming the microtubule net below the surface of the ventral cortex. These results indicate that the basal components of the ciliature cyto6keleton and the ciliature base-associated microtubules of P. weissei are tubulins. Besides this, the constructive character of marginal cirri base-asseciated microtubules of P. weissei were different from those of other ciliates （ e. g. Stylonychia mytilus ）, whose TM of left marginal cirri protruded from the ciliature and extended to the right area. During the process of morphogenesis, part of the old AZM was renewed, and the AZM of the proter was formed at the site where the old one was located. The old marginal cirri played an important part in the orientation of the new marginal cirri and supplied its development with some substances. After that, the new marginal cirri formed at the right area but not the original area of the old ones. In some cells, one row of cirri appeared at the left of the new left marginal cirri, it may be formed because the old left marginal cirri had degenerated incompletely .

关 键 词：魏氏拟尾柱虫 皮层 纤毛器 微管胞器 形态发生 

分 类 号：Q954.4[生物学—动物学]