驱蚊草组织培养及其愈伤组织诱导研究  

Research on tissue culture and callus induction of Mozzie buster

作　　者：吴林[1] 吴飞[1] 余家平[1] 蒋琳[1] 张晨晨[1] 王钰[1] 

机构地区：[1]安徽大学生命科学学院安徽省生态工程与生物技术重点实验室,合肥230039

出　　处：《生物学杂志》2007年第4期45-48,共4页Journal of Biology

基　　金：安徽省人才基金项目;安徽省教委项目

摘　　要：运用正交设计方法,利用植物组织、细胞培养技术,成功地建立了驱蚊草组织培养快繁技术体系。研究出:不定芽诱导的最适培养基为MS+6-BA0.2mg/L+NAA0.3mg/L,增殖倍数为6.73;生根培养基为1/2MS+NAA0.4mg/L,平均每株生根数为11.2条,生根率为90.0%。通过对其离体茎段的培养研究实验,得出驱蚊香草的最适愈伤组织诱导培养基为MS+2,4-D0.5mg/L+6-BA2.0mg/L+NAA0.3mg/L,发愈率为93.3%,愈伤组织多为淡黄色,质地疏松。Based on taking the orthogonal design experiments and by using the technique of plant tissue culture, the experiment - system of tissue culture and rapid propagation of Mozzie buster was successfully established. The result showed that ： the most suitable medium for the initiation of adventitious buds was MS medium supplemented with 0. 2 mg/L 6 - BA and 0.3 mg/L NAA, the ratio of buds propagation was 6. 73 ; The best medium for the rooting of shoots was 1/2 MS medium supplemented with 0. 4 mg/L NAA , the average number of roots per shoot was 11.2 and the rate of rooted shoots was 90. 0%. The culture of the detached stems of Mozzie buster plantlets was studied, Result showed that the optimal medium for the inducement of callus tissues was MS medium supplemented with 0. 5 mg/L 2 , 4 - D, 2.0mg/L 6 - BA and 0. 3mg/L NAA , the rate of callus tissue inducement was 93.3% and the callus tissues were mostly yellowish and loose in texture.

关 键 词：正交设计 驱蚊草 组织培养 愈伤组织 培养基 

分 类 号：Q943.1[生物学—植物学]