不同色谱柱和GPC软件对灰树花倍他葡聚糖相对分子质量测定的影响  

Influence of different chromatograph columns and GPC softwares on relative molecular mass determination of Grifola frondosa beta-glucan

作　　者：赵峡[1] 杨海[1] 宋乐天[1] 于广利[1] 徐泽平 

机构地区：[1]中国海洋大学海洋药物与食品研究所,青岛266003 [2]山东仁和生物有限公司,博兴256500

出　　处：《药物分析杂志》2007年第8期1143-1146,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目资助(30370345)

摘　　要：目的:考察不同的色谱柱和 GPC 软件对灰树花倍他葡聚糖相对分子质量(M_r)测定的影响。方法:分别采用 TSKG4000PW_(XL)和 Shodex OHpak SB-804HQ 色谱柱按高效凝胶渗透色谱法对灰树花倍他葡聚糖的 M_r 进行测定,再分别采用安捷伦公司、岛津公司、龙智达公司和千谱公司的 GPC 专用软件进行计算,并对各 M_r 计算结果进行比较。结果:采用 TSKG4000PW_(XL)和 Shodex OHpak SB-804HQ 色谱柱测定灰树花倍他葡聚糖的 M_r 结果有明显差异,其重均相对分子质量(M_w)相差>10%,峰位相对分子质量(M_p)相差>20%,但 M_r 的分布宽度(D)无明显差异;采用4种不同公司的 GPC 软件进行计算的M_r 结果相差较小,其相对偏差<3%。结论:不同的色谱柱对灰树花倍他葡聚糖 M_r 的测定结果有明显影响,但不同 GPC 软件对 M_r 的测定结果影响较小。Objective：To investigate the influence of different chromatograph columns and GPC softwares on relative molecular mass Mr determination of Grifola frondosa beta- glucan （GFG）. Methods：Determine Mr of GFG by High performance gel permeation chromatogram （HPGPC） with Shodex OHpak SB -804HQ and TSK G4000PWxL columns respectively, and then compare the results of Mr of GFG,which calculated by softwares of Aglient, Shimadzu, Lonzhida and Qianpu GPC softwares, respectively. Results：There are obvious differences between results of Mr determined by Shodex OHpak SB -804HQ and TSK G4000PWxL columns. The deviation of relative weight average molecular mass （Mw） and peak molecular mass （Mp） are above 10% and 20% respectively, but the molecular mass distribution width （D） between two columns are similar. There is little difference between the molecular mass calculated by four different GPC softwares ,and their relative deviations are less than 3%. Conclusion： Different chromatograph columns have notably influence and different GPC softwares have little influence on Mr determination of GFG.

关 键 词：灰树花倍他葡聚糖 高效凝胶渗透色谱法 GPC软件 相对分子质量 

分 类 号：R917[医药卫生—药物分析学]