HPLC法同时测定白花蛇舌草注射液中2种成分的含量  

Simultaneous HPLC determination of two components in Baihua Sheshecao injection

作　　者：郭云珍[1] 宝炉丹[1] 潘永玉[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2007年第8期1158-1160,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立反相高效液相色谱法同时测定白花蛇舌草注射液中对香豆酸(Ⅰ)和反式6-O-对香豆酰鸡屎藤苷甲酯(Ⅱ)的含量。方法:色谱柱为 Hypersil C_8柱(150 mm×4.6 mm,5μm);流动相为乙腈-水(20:80,v/v),含0.3%醋酸;流速为1.0 mL·min^(-1);检测波长为310 nm。结果:Ⅰ和Ⅱ的浓度分别在14.4～115.2μg·mL^(-1)(r=0.9999)和5.2～41.4μg·mL^(-1)(r=0.9999)范围内线性关系良好;方法回收率分别为99.9%(RSD=1.5%,n=6)和99.8%(RSD=1.8%,n=6)。结论:本法准确可靠,可用于白花蛇舌草注射液的质量控制。Objective ：To establish RP- HPLC method for simultaneous determination of p -coumaric acid （ Ⅰ ） and E-6 -O -p -coumaroyl scandoside methyl ester （Ⅱ ） in Baihua Sheshecao injection. Methods：The separation was performed on a Hypersil Cs column （ 150 mm ×4. 6 mm,5μm） with acetonitrile - water （20：80 ,v/v,containing 0.3% acetic acid） as the mobile phase at the flow late of 1.0 mL·min^-1. The UV detection was set at 310 nm. Results：There was good linearity between the peak area and concentration at the ranges of 14. 4 - 115.2μg· mL ^- 1 （ r = 0. 9999 ） and 5.2 - 41.4μg· mL ^- 1 （ r = 0. 9999 ） for Ⅰ and Ⅱ , respectively. The average recoveries of Ⅰ and Ⅱ were 99.9% （ RSD = 1.5%, n = 6 ） and 99.8% （ RSD = 1.8%, n = 6 ）, respectively. Conclusion. Themethod is accurate for the quality control of Baihua Sheshecao injection.

关 键 词：HPLC 白花蛇舌草注射液 对香豆酸 反式6-O-对香豆酰鸡屎藤苷甲酯 

分 类 号：R917[医药卫生—药物分析学]