RP-HPLC法测定人体血浆中芬布芬的浓度  

RP-HPLC determination of fenbufen in human plasma

作　　者：谷艳[1] 王静[1] 赵春杰[1] 

机构地区：[1]沈阳药科大学,沈阳110016

出　　处：《药物分析杂志》2007年第8期1197-1199,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定人血浆中芬布芬浓度的高效液相色谱法。方法:采用 Diamonsil(钻石)ODS C_(18)色谱柱(250 mm×4.6mm,5μm),以甲醇-0.02 mol·L^(-1)磷酸二氢钾-乙腈(75:20:5)(用磷酸调节 pH 3.0)为流动相,流速为1.0 mL·min^(-1),检测波长285 nm。结果:芬布芬的线性范围为0.16～16.0μg·mL^(-1),定量限为0.16μg·mL^(-1),检测限为0.06μg·mL^(-1),平均回收率为(101.2±3.5)%,RSD 为1.8%,日内及日间精密度分别为1.7%和2.7%。结论:该方法简便、快速、准确,适用于临床上芬布芬的体内研究。Objective：To establish an RP- HPLC method for the determination of fenbufen in human plasma. Method：Separation was achieved on a Diamonsil ODS C18 column （250mm×4.6mm,5μm） and detected at 285 nm. The mobile phase was a mixture of methanol -0.02 mol· L^-1 KH2 PO4 - acetonitrile （75：20：5 ） （ pH was adjusted to 3.0 by phosphoric acid） at a flow rate of 1.0 mL·min^-1. Results：The linear range was 0. 16 - 16. 0 μg mL·min^-1. The limit of quantification was 0. 16 μg·mL^-1 The limit of detection was 0. 06 μg·mL^-1. The average recovery was （ 101.2 ± 3.5 ） % with RSD of 1.8%. The intra - day precision was 1.7% and the inter - day precision was 2. 7%. Conclusion：The method is simple, quick,accurate, and is suitable for the determination of fenbufen concentration in human plasma and pharmacokinetic studies.

关 键 词：高效液相色谱法 芬布芬 人血浆药浓度 药动学 

分 类 号：R917[医药卫生—药物分析学]