HPLC法测定β-榄香烯脂质体药物含量及包封率  

HPLC determination of the content and entrapment efficiency of β-elemene liposome

作　　者：宋笑丹[1] 王学军[2] 杜霞[1] 董迪[1] 吴琳华[1] 

机构地区：[1]哈尔滨医科大学附属第二医院药学部,哈尔滨150086 [2]黑龙江省中医研究院,哈尔滨150036

出　　处：《药物分析杂志》2007年第8期1222-1224,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立β-榄香烯脂质体药物含量及包封率测定的 HPLC 法。方法:采用 Diamonsil C_(18)(4.6 mm×200 mm,5μm)色谱柱,流动相为乙腈-水(80:20),流速1.0 mL·min^(-1),检测波长210 nm,柱温25℃,进样量20μL。采用葡聚糖凝胶(Sephadex G-50)柱分离脂质体中游离药物。结果:在本色谱条件下β-榄香烯与辅料及溶剂峰分离良好,β-榄香烯浓度在5.0～60.0μg·mL^(-1)范围内与峰面积呈良好的线性关系(r=0.9994),精密度试验的 RSD 为1.4%～4.2%(n=5),重复性试验的 RSD 为3.1%(n=5),平均回收率为101.8%(n=15)。结论:该方法简便、易行,可用于β-榄香烯脂质体含量及包封率的测定。Objective：To establish an HPLC method for determining the content and entrapment efficiency of β- elemene liposome. Methods：HPLC analysis was performed on a Diamonsil Cls column （4.6 mm ×200 mm,5 μm） with mobile phase of acetonitrile -water （80： 20） at the flow rate of 1.0 mL· min^-1. The injection volume was 20μL. The UV detection wavelength was set at 210 nm. The column compartment temperature was set at 25℃. For assay of liposome entrapment efficiency, Sephadex G - 50 was used for removing the free β- elemene before sample preparation of HPLC analysis. Results ：β- elemene had a good linear relation in the range of 5.0 - 60. 0 μg·mL^- 1 （r =0. 9994）. The precision （RSD） was 1.4% -4. 2% （n =5） ,the reproducibility （RSD） was 3.1% （n =5）, the average recovery rate was 101.8% （n = 15 ）. Condusion：The developed method is simple ,accurate and reliable for determination of the content and entrapment efficiency of β-elemene liposome.

关 键 词：Β-榄香烯脂质体 HPLC 含量测定 包封率 

分 类 号：R917[医药卫生—药物分析学]