RP-HPLC测定更舒安颗粒中马钱苷含量  

RP-HPLC determination of loganin in Gengshu'an granules

作　　者：陈卫东[1] 黄轶君[1] 葛秀文[1] 吴萍[1] 惠永正[1] 王骏[2] 徐宏喜 

机构地区：[1]上海中药创新研究中心,上海201203 [2]香港中文大学 [3]香港赛马会中药研究院

出　　处：《药物分析杂志》2007年第8期1243-1245,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：香港赛马会慈善基金资助

摘　　要：目的:建立反相高效液相色谱法测定更舒安颗粒中马钱苷含量。方法:色谱柱采用 Agilent ZORBAX SB C_(18)(4.6 mm×250 mm,5μm);以乙腈-水(15:85)为流动相,流速1.0 mL·min^(-1);柱温25℃;于240 nm 波长下检测。结果:在测定条件下,马钱苷在29.6～296.0μg·mL^(-1)浓度范围内呈良好的线性(r=0.9999),最低检出浓度为0.1μg·mL^(-1)。低、中、高3种浓度的方法回收率分别为100.5%,100.2%,101.3%,RSD(n=3)均小于2%。结论:本方法准确、简便、专属性强、灵敏度高,结果稳定,适用于更舒安颗粒的质量控制。Objective：A RP - HPLC method was established for the determination of loganin in Gengshuan granules. Methods：The RP - HPLC method was performed with a Agilent ZORBAX SB C18 column （4. 6 mm 250 mm, 5 μm） at 25 ℃ ,and the mobile phase was acetonitrile-water （15：85 ,v/v）mixture. The flow rate was 1.0 mL· min^-1. The loganin was detected by UV at 240 nm. Results： The linear range of the loganin was 29.6 - 296.0 μg·mL^-1 （ r = 0. 9999 ）, and the limit of detection was 0. 1 μg·mL^-1. The method recoveries were 100. 5% , 100.2% and 101.3 % , and RSD （ n = 3 ） for low, medium, high were less than 2%. Conclusion： The method is simple, sensitive, selective and repetitive, and is suitable for quality control of Gengshu＇ an granules.

关 键 词：马钱苷 高效液相色谱法 颗粒 

分 类 号：R917[医药卫生—药物分析学]