反向毛细管电泳高频电导法测定牛黄中的胆酸  

Rapid and simple determination of choicacid in bezoar by reversed-capillary electrophoresis with high frequency conductivity detection

作　　者：周勰[1] 吕途[2] 陈缵光[1] 张菁[2] 

机构地区：[1]中山大学药学院,广州510089 [2]中山大学中山医学院,广州510089

出　　处：《分析试验室》2007年第5期5-7,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20375049);广东省自然科学基金(021808)项目资助

摘　　要：建立了毛细管电泳高频电导法快速简便测定牛黄粉中胆酸的新方法.考察了电泳介质的种类、浓度、 pH值以及操作电压和进样时间对分离检测的影响.缓冲液为0.5 mmol/L Na3PO4＋2 mmol/L Na2HPO4＋0.2 mmol/L CTAB（pH 11.7）, 分离电压为 -15 kV时可实现较好的分离和检测.胆酸的线性范围为75～200 μg/mL, 检出限为0.1 μg/mL.线性方程为y=0.6054ρ-4.14991, r=0.953.回收率为100.9%, RSD（n=5）=2.1%.A rapid and simple method to determine choicacid in Bezoar by reversed-capillary electrophoresis with high frequency conductivity detection was described. In the buffer of 0.5 mmol/L Na3PO4 ＋ 2 mmol/L Na2HPO4 ＋ 0.2 mmol/L CTMAB （pH 11.7） at separation voltage of 15.0 kV, the choicacid in Bezoar could be separated and detected well. Under the chosen conditions, the linear concentration of choicacid ranged from 75 to 200μg/mL, the limit of detection was 0.1 μg/mL. The method could be used for analysis of choicacid in bezoar with a recovery of 100.9%. The method is rapid and simple for the determination of choicacid.

关 键 词：反向毛细管电泳 高频电导检测 非接触式电导检测 牛黄 胆酸 

分 类 号：O657.8[理学—分析化学]