同步荧光法测定人尿中异黄蝶呤  

Determination of isoxanthopterin in human urine by synchronous fluorescence scan

作　　者：万益群[1] 谭婷[1] 郭岚[1] 

机构地区：[1]南昌大学分析测试中心,南昌330047

出　　处：《分析试验室》2007年第5期8-11,共4页Chinese Journal of Analysis Laboratory

基　　金：江西省自然科学基金(0520057)项目资助

摘　　要：研究了一种测定人尿中异黄蝶呤的同步荧光分析方法.在KH2PO4-NaOH缓冲溶液中（pH 7.8）,波长差（Δλ）为65 nm的条件下进行同步荧光扫描,可有效排除尿液中其它物质对异黄蝶呤测定的干扰.异黄蝶呤的质量浓度在0～1.4 μg/mL范围内与荧光强度呈良好的线性关系.线性方程为Ⅰ=4105.3ρ＋59.696, 相关系数r=0.9997.异黄蝶呤添加到健康人和胃癌病人尿样中的平均回收率分别在102.9%～108.4%及85.6%～95.6%之间,其相对标准偏差（RSD）分别为0.37%～1.2%及1.0%～2.2%.方法已应用于人尿中异黄蝶呤的测定.A synchronous fluorescence method was studied to determine the isoxanthopterin in human urine. The measurement was carried out in a KH2 PO4-NaOH buffer solution （pH 7.8） with △λ = 65 nm for synchronous scanning, which can exclude interferences of other substances in urine samples. The linear range for the concentration of isoxanthopterin was found to be 0- 1.4 μg/mL. The linear regression equation was I = 4105.3p 4-59.696, with linear correlation coefficient of 0.9997. Both healthy people＇s urine samples and patients＇ urine samples were measured. For healthy people, the recoveries were in the range of 102.9% - 108. %, the relative standard derivations （RSD） were 0.37% - 1.2%, while for the patients＇ recoveries were in the range of 85.6% - 95.6%, and the RSD were 1.0% -2.2%. The proposed method was successfully applied to the determination of isoxanthopterin in human urine samples.

关 键 词：异黄蝶呤 尿液 同步荧光 

分 类 号：O657.34[理学—分析化学]