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机构地区:[1]涪陵师范学院化学及环境科学系,重庆408003 [2]涪陵广播电视大学,重庆408000
出 处:《分析试验室》2007年第5期19-22,共4页Chinese Journal of Analysis Laboratory
基 金:重庆市教委科技基金(KJ051304)项目资助
摘 要:研究了茜素与麦迪霉素(MID)、红霉素(ERY)的显色反应.在pH 5.5~6.8的BR缓冲溶液中,茜素与麦迪霉素(MID)、红霉素(ERY)反应生成红色络合物.最大吸收波长分别为528 nm (MID)和534 nm (ERY);表观摩尔吸光系数为8.3×10^3 L·mol^-1·cm^-1 (MID)和1.01×10^4 L·mol^-1·cm^-1(ERY);麦迪霉素质量浓度在0~16.5 mg/L和红霉素质量浓度在0~18.0 mg/L范围内均符合比耳定律,据此建立了测定麦迪霉素和红霉素的分光光度法,并探讨了适宜的反应条件.该法可用于麦迪霉素、红霉素药物的测定.The colour reactions of alizarin (ALZ) with midecamycin (MID) and erythromycin (ERY) were investigated. In the BR medium at pH 5.5-6.8 alizarin (ALZ) reacts with midecamycin (MID) or erythromycin (ERY) to form red compounds. The maximum absorption wavelengths am in 528 nm (MID) and 534 nm (ERY) ; their apparent molar absorptivities (ε) were 8.3 ×10^3 L·mol^-1·cm^-1 (midecamycin) and 1.01 ×10^4 L·mol^-1·cm^-1 (erythromycin); Beer's law is obeyed in the range of 0 - 16.5 mg/L for midecamycin and in the range of 0 - 18.0 mg/L for erythmmycin. A new spectmphotometric method for the determination of midecamycin or erythromycin has been developed. The optimum reaction conditions were studied. The method was applied to determination of the contents of midecamycin and erythromycin sold in the cities.
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