反相高效液相色谱法测定人尿中利凡诺  

Determination of rivanol in Human urine samples by RP-HPLC

作　　者：郭智勇[1] 魏丹毅[1] 储成顶[2] 尹耕心 赵莎莎[3] 

机构地区：[1]宁波大学材料科学与化学工程学院宁波市新型功能材料及其制备科学实验室-省部共建国家重点实验室培育基地,宁波315211 [2]安徽医科大学公共卫生学院,合肥230032 [3]安徽省计划生育研究所,合肥230031

出　　处：《分析试验室》2007年第5期58-61,共4页Chinese Journal of Analysis Laboratory

基　　金：安徽省人口和计划生育委员会专项基金;安徽省教育厅科研基金(2005KJ251);浙江省科技厅科技攻关(2006C33065)项目资助

摘　　要：建立了运用反相高效液相色谱法测定人尿中利凡诺的方法。采用Spher-isorb C18色谱柱(250 mm×4.6 mm i.d.,5μm);流动相:V(甲醇)∶V(乙腈)∶V(0.2 mol/L NH4Ac)=60∶37∶3;流速:1.0 mL/min;柱温:40℃;荧光检测器:激发波长360 nm,发射波长500 nm。在5 ng/mL^1μg/mL质量浓度范围内,呈现良好线性(r=0.9999),检出限为1 ng/mL。A RP-HPLC method for determining rivanol in human urine samples was established. Rivanol was chromatographod on a Spherisorb C18 column （250 mm× 4.6 mm, 5μm） with the column temperature of 40 ℃. The mobile phase used was methanol-acetonitrile-NH4Ac （0.2 mol/L） （60：37：3）, with the flow rate of 1.0 mL/min. Fluorimettic detector was used with the excitation wavelength at 360 nm and the emission one 500 nm. The standard curve was linear in the range of 5 ng/mL- 1μg/mL with the correlation coefficient 0.9999. The limit of detection was 1 ng/mL. The proposed method is simple, highly accurate and reliable.

关 键 词：反相高效液相色谱法 利凡诺 尿样 

分 类 号：O657.72[理学—分析化学]