N-乙酰氨基葡萄糖水溶液变旋过程的液质联用和旋光研究  

Study on mutarotation of N-acetylglucosamine in water by HPLC/MS/MS and specific rotation

作　　者：李晓东[1] 仲宣惟[2] 张雅军[1] 尹利辉[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]国家食品药品监督管理局,北京100810

出　　处：《分析试验室》2007年第5期112-114,共3页Chinese Journal of Analysis Laboratory

摘　　要：研究N-乙酰氨基葡萄糖在水溶液中的变旋行为。通过液质联用及比旋度分析,监测N-乙酰氨基葡萄糖水溶液的变旋情况。得到基于APCI离子源的N-乙酰氨基葡萄糖的多级质谱图,确认其水溶液变旋稳定时间及稳定状态下的差向异构体组成比例,并对N-乙酰氨基葡萄糖HPLC图中的差向异构体色谱峰进行指认。为存在变旋行为的糖及糖苷类化合物的研究提供了准确可行的方法。To mutarotation of N-acetylglucosamine in water was studied by HPLC/MS/MS with specific rotation. The MS/MS and MS spectra of N-Acetylglucosamine was performed by APCI probe. The anomers, final equilibrium time and ratio were confirmed by HPLC/MS/MS and specific rotation. The study has provided available and reliable method for the mutarotation of water.

关 键 词：N-乙酰氨基葡萄糖 变旋 液质联用 比旋度 

分 类 号：TQ460.5[化学工程—制药化工]