重组异体脱蛋白骨关节移植修复骨关节缺损的实验研究  

Experimental study on treatment of osteoarticular defects with recombinant deproteinized osteoarticular allograft

作　　者：瞿向阳[1] 蒋电明[1] 安洪[1] 

机构地区：[1]重庆医科大学第一附属医院骨科,400016

出　　处：《中华创伤杂志》2007年第8期605-608,共4页Chinese Journal of Trauma

基　　金：重庆市科技攻关资助项目（渝科发计字200138号）

摘　　要：目的 探讨钙磷骨水泥（CPC）复合重组人血管内皮细胞生长因子（rhVEGF）＋重组人骨形态发生蛋白-2（rhBMP-2）与异体脱蛋白骨（DPB）关节移植修复骨关节缺损的能力。方法 成年杂交犬36只，随机分成3组：A组为CPC／rhVEGF／rhBMP-2／DPB关节移植；B组为rhVEGF／rhBMP-2／DPB关节移植；C组为单纯的DPB关节移植。术后观察血管发生和新骨形成情况。结果 A组在各时相点再血管化、成骨能力和软骨形成检测结果均优于B、C两组，B、C组间在各时相点差异无统计学意义。结论 重组脱蛋白骨关节材料具有较强的再血管化和成骨能力，可早期与受体达到骨愈合修复骨关节缺损并最终成为自身组织。Objective To compare the ability of calcium phosphate cement （CPC） combined with recombinant human vascular endothelial growth factor （rhVEGF） plus recombinant human bone morphogenetic protein-2 （rhBMP-2） plus osteoarticular deproteinized bone （DPB） allograft in repairing osteoarticular defects. Methods A total number of 36 adult hybrid dogs were divided randomly into 3 groups, ie, Group A （CPC/ rh-VEGF/rh-BMP-2/DPB was transplanted） , Group B （rhVEGF/rhBMP-2 /DPB was transplanted） and Group C （only DPB was transplanted）. After operation, vasculogenesis and new bone formation were observed. Results The revascularization, new bone formation and chondrogenesis at 4 time points after operation in Group A were found significantly superior to those in Groups B and C. But no statistically significant difference was found between Group B and Group C. Conclusions The recombinant deproteinized osteoarticular allograft has a fairly excellent ability to promote new bone formation and vasculogenesis, achieve early bone healing and osteoarticular defect repair and ultimately become autologous tissue.

关 键 词：骨移植 血管内皮细胞生长因子 骨形态发生蛋白质类 再血管化 

分 类 号：R686[医药卫生—骨科学]