山羊抑制素α亚基基因HaeⅡ酶切多态性及其与产羔数性状的关联分析  

HaeⅡ RFLP of INHA and its relationship to goat litter size

作　　者：滑国华[1] 陈世林[1] 姚红卫[1] 吴伟生[1] 沈忠[2] 陈启康 陈林 闻群英[5] 杨利国[1] 

机构地区：[1]华中农业大学动物科技学院,武汉430070 [2]湖北省畜牧局,武汉430070 [3]江苏省南通市农业科学研究所,南通226541 [4]湖北省房县畜牧局,房县442100 [5]湖北省十堰市畜牧局,十堰442100

出　　处：《遗传》2007年第8期972-976,共5页Hereditas（Beijing)

基　　金：国家农业成果转化资金项目(编号:05EFN214200186);华中农业大学创新基金项目(项目编号:52204-05015)资助~~

摘　　要：以323只马头山羊、努比山羊、波尔山羊和海门山羊为试验材料,利用PCR-SSCP、PCR-RFLP和克隆测序等方法对山羊抑制素α亚基基因(INHA)编码区一个片段进行多态性分析,发现在该基因第284位(登录号:L28815)存在G→A突变,该突变引起HaeⅡ酶切位点改变;HaeⅡ-RFLP分型结果显示G等位基因为优势等位基因,INHA不同基因型平均产羔数表现为GG>AG>AA。结果显示:INHA可能是影响山羊产羔数性状的一个主效基因,G等位基因可能与高产性状呈正相关。The polymorphism in a fragment within the coding region of the inhibin α subunit （ INHA ） gene was studied in 323 heads of Matou, Nubi, Boar and Haimen goats by PCR-SSCP, PCR-RFLP and sequencing. A new mutation G284A （Accession number： L28815） was identified, which could be detected by Hae Ⅱ digestion of a PCR product spanning this site. Hae Ⅱ PCR-RFLP analysis indicated that allele G was dominant. Association studies indicated that the effect of INHA genotypes on litter size was greatest for GG, followed by AG and AA genotypes. Thus, INHA may be a major gene controlling the prolificacy of goat, and allele G is positively correlated with litter size.

关 键 词：山羊 INHA 多态性 产羔数 

分 类 号：S827[农业科学—畜牧学]