SDS增敏荧光光度法测定人血清中左氧氟沙星的残留量  

Rapid determination of Levofloxacin residues in person serum by SDS-fluorescence spectrometry

作　　者：董学芝[1] 席会平[1] 胡卫平[1] 李畅[1] 张国胜[1] 

机构地区：[1]河南大学化学化工学院,河南开封475001

出　　处：《华西药学杂志》2007年第4期435-437,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立人血清中左氧氟沙星(LOX)残留量的快速测定方法。方法采用十二烷基硫酸钠(SDS)增敏荧光光度法。结果用无水乙醇沉淀蛋白,对血清进行预处理,利用LOX-SDS体系测定,人血清中LOX在0.1～5.0μg.ml-1时,荧光强度与浓度呈线性关系,其回归方程为:F=5.552+40.019C(r=0.9990),平均回收率为99.72%,RSD=0.68%。结论所建方法简便、准确、快速,可用于人血清中LOX的测定。OBJECTIVE To establish a method for quantitative determination of Levofloxacin（LOX） in hunan serum. METHODS LOX was determined by SDS - fluorescence spectrophotometry. RESULTS Absolute alcohol was used to remove protein in the serum. And in the LOX -SDS system, the linear range was 0. 1 -5.0 μg·ml^-1 and regression equation was F =5. 552 ＋40. 019C（r = 0. 9990）. The average recovery of LOX was 99.72% with the RSD of 0. 68%. CONCLUSION This method is simple, accurate, rapid and can be used to determine LOX in human erum.

关 键 词：十二烷基硫酸钠增敏荧光分光光度法 左氧氟沙星 血清 残留量 

分 类 号：R917[医药卫生—药物分析学]