LC-MS/MS测定人血浆中的辛伐他汀  

Determination of Simvastatin in human plasma by LC-MS/MS

作　　者：刘拥军[1] 栾杨[1] 王秀丽[1] 

机构地区：[1]山东省医学科学院药物研究所,山东济南250062

出　　处：《华西药学杂志》2007年第4期439-441,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立测定人血浆中辛伐他汀的LC-MS/MS方法。方法血浆样品经乙酸乙酯提取后,以乙腈-水(含0.1%乙酸和2.5 mmo.lL-1甲酸胺)(90:10)为流动相,通过Agilent1100 VL型离子阱质谱仪,电喷雾离子源正离子模式,采用多反应离子监测方式测定辛伐他汀的浓度(MRM,m/z441→325)。结果血浆中辛伐他汀的线性范围为0.5～10.0 ng.ml-1。定量下限为0.5 ng.ml-1。平均回收率85%～115%,日内、日间精密度在±15%之内。结论所用方法高效、准确,特异性强,可用于辛伐他汀的药物动力学研究。OBJECTIVE To establish an LC - MS/MS method to determine Simvastatin in human plasma. METHODS Plasma samples were extracted by ethyl acetate,and then separated on a Zorbax SB -C18 （150 mm × 4. 6 mm）column with acetonitrile-H2O （containing 0. 1% HAc ＋ 2. 5 mmol·L^-1 ammonium formate） （90 ： 10 ）as mobile phase. Electrospray ionization source（ESI） was applied and operated in positive ion mode. Multiple reactions monitoring （MRM） mode was used and Simvastatin was determined by monitoring the ion transition of m/z 441→325. RESULTS The linear cafibration curves were obtained over the concentration range of 0. 5 - 10. 0 ng·ml^-1 in plasma. The lower limit of quantitation was 0. 5 ng·ml^-1. Recovery was within 85% - 115% ,the within - day and day -to -day precision were within ± 15%. CONCLUSION The method proved to be effective,accurate and specific,with can be applied to the pharmacokinetie study of simvastatin.

关 键 词：辛伐他汀 LC-MS/MS 血浆样品 

分 类 号：R917[医药卫生—药物分析学]