前胡甲素上调实验性自身免疫心肌炎巢蛋白表达  

Praeruptorin A upregulates expression of nestin in experimental autoimmune myocarditis of rats

作　　者：林娜[1] 徐赫童[1] 杜子婧[1] 王学范[1] 常天辉[1] 

机构地区：[1]中国医科大学药学院临床药理教研室,沈阳110001

出　　处：《中药药理与临床》2007年第3期21-23,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：卫生部重点基金资助课题;№88402257

摘　　要：目的:研究前胡甲素(Pd-Ia)对心肌炎中间丝巢蛋白表达的影响及其心肌保护作用。方法:采用大鼠实验性自身免疫心肌炎(experimental autoimmune myocarditis,EAM)模型,通过Western blot法检验巢蛋白的表达,同时测量其心重/体重比,并对血液和心肌组织做心肌酶谱和病理学分析。结果:Pd-Ia能增加巢蛋白表达,减轻心肌损伤。结论:Pd-Ia能上调巢蛋白表达,起到心肌保护作用。Objective： To investigate the influence of dl- praeruptorin A （Pd- Ia） on expression of intermediate filament protein nestin and its cardioprotective actions in myocarditis. Methods： Effect of Pd - Ia on expression of nestin was evaluated in rats that had recovered from experimental autoimmune myocarditis （EAM）. Wistar rats were divided into 5 groups, including group sham, group natural saline, group solvent control （polyethylene glycol） , group Pd- Ia 1.0 mg/kg, and group positive control （verapamil0.5 mg/kg）, respectively. Thereafter, all animals were immunized with pig cardiac myosin on day 0. Drugs were administered twice through abdominal cavity on day 20 and day 21, respectively. Four hours after second administration, rats are executed. Expression of nestin in myocardium was detected by Western blot analysis. Heart weight/body weight ratio, myocardial enzyme spectrum, and histopathology were measured simultaneously. Results： Pd -Ia upregulated expression of nestin and relieved myocardial injury in EAM of rats. Values of relative optic density in Pd - Ia（ 1.0 mg/kg） group were increased from 37.5±3 to 61±4 （ P 〈 0.05, vs solvent, n = 5）. Conclusion： Pd - Ia was beneficial to facilitate nestin expression in myocarditis, and suitable in treatment of early myocarditis.

关 键 词：前胡甲素 中间丝蛋白 巢蛋白 心肌炎 

分 类 号：R285.5[医药卫生—中药学]