槲芪散及其君药槲寄生提取物对人肝癌细胞生长和端粒酶活性的影响  

Effect of Huqi San(槲芪散) and mistletoe on human hepatocarcimoma cells and telomerase activity

作　　者：刘树红[1] 吕喆[2] 李霞[1] 丰平[1] 孔庆利[2] 孙海梅 王学江[1] 

机构地区：[1]首都医科大学病理生理教研室,北京100069 [2]首都医科大学免疫教研室,北京100069 [3]不详

出　　处：《中药药理与临床》2007年第3期62-65,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：北京市中医药科技发展基金(NoJJ2005-10)课题;北京市教委科技发展项目基金(NoM200610025003)课题

摘　　要：目的:研究槲芪散及其君药槲寄生对人肝癌细胞生长和端粒酶活性的影响。方法:用不同浓度的槲芪散及其君药槲寄生提取物作用于人肝癌细胞(SMMC-7721)后,用四甲基偶氮唑比色法(MTT)检测SMMC-7721细胞增殖。用实时荧光定量PCR法检测SMMC-7721的端粒酶活性。结果:(1)MTT结果显示槲芪散、槲寄生多糖和总碱能抑制SMMC-7721细胞,随药物作用时间的延长作用增强。(2)槲芪散、槲寄生多糖和槲寄生总碱能明显降低SMMC-7721端粒酶的活性。结论:槲芪散及槲寄生多糖和总碱均能抑制SMMC-7721增殖,降低SMMC-7721端粒酶的活性。Objective： To study the effect on human hepatocarcinoma cells and telomerase activity induced by Huqi San （HQS） and its principal drug mistletoe extracts Methods： After the SMMC -7721 induced by different concentration, we select the MTT to detect the proliferation of SMMC-7721; use the fluorescent quantitative PCR to detect the telomerase activity of SMMC -7721. Results： （1）The results of MTT showed that compared to control group, the inhibition ratio of SMMC -7721 induced by the low -dose mid -dose and high -dose of HQS, mistletoe polysaccharides and total alkaloid of mistletoe increase gradually. （2） The low - dose mid - dose and high - dose of HQS, mistletoe polysaccharides and total alkaloid of mistletoe have the effect of degrading the telomerase activity of SMMC -7721 compared to normal control group and C - DDP （ cis - diamminedichloroplatinum） group. Conclusions： Huqi San （HQS） and its principal drug mistletoe polysaccharides and total alkaloid of mistletoe can all inhibit the proliferation of SMMC -7721 and degrade the telomerase activity of SMMC -7721.

关 键 词：槲芪散 槲寄生 端粒酶 MTT 实时PCR SMMC-7721 

分 类 号：R285.5[医药卫生—中药学]