高效液相色谱法测定人血浆中司帕沙星药物浓度  

Determination of the content of Sparfloxacin in human plasma by HPLC

作　　者：罗继名[1] 张永东[1] 肖云彬[1] 林杰[1] 李嫩祥 

机构地区：[1]郴州市第一人民医院,湖南郴州423000 [2]浏阳市人民医院,长沙410300

出　　处：《实用药物与临床》2007年第4期252-253,共2页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立测定人血浆中司帕沙星的药物浓度的方法。方法采用乙腈萃取,高效液相色谱法测定。色谱柱：Hypersil BDS-C18（4.6 mm×150 mm,5μm）;流动相：0.25%磷酸乙腈溶液（450∶100,用三乙胺调pH=2.50）;流速：1 mL/min;紫外检测器：波长298 nm,柱温40℃。结果司帕沙星的保留时间为6.1 min,定量线性范围：0.06-2.16μg/mL,方法回收率〉90%（n=5）,日内、日间RSD〈10%（n=5）。结论本方法快速,定量准确,可用于血药浓度测定及人体药代动力学研究。Objective To determine the Sparfloxacin concentration in human plasma. Methods The plasma sample was extracted by acetonitrile and determined by HPLC, the stationary phase was Hypersil BDS-C18 （4.6 mm× 150 mm, 5 μm）, the mobile phase consisted of 0.25 % H3PO4 and acetonitrile （450 ： 100）, pH = 2.50 by spasmolytol. The flow rate was 1.0 mL/min, ultraviolet detector was performed with wavelength detector at 298 nm. Results The retention time of Sparfloxacin was about 6.1 min, and the linear range of quantity was 0.06- 2.16 btg/mL. The recoveries of method were more than 90 % （ n = 5）, RSD 〈 10 % （ n = 5）. Conclusion This method is rapid and accurate, It can be used for the determination of the content of plasma Sparfloxacin and the study of pharmacokinetics.

关 键 词：司帕沙星 血药浓度 高效液相色谱法(HPLC) 

分 类 号：R96[医药卫生—药理学]